Literature DB >> 7092140

A simple automated colorimetric method for determination of N-acetyl- beta-D-glucosaminidase.

J C Moore, J E Morris.   

Abstract

An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variation 1.2% and inter-assay coefficient of variation 1.6%. Inhibition of enzyme activity by heparin in plasma samples can be reversed by the addition of calcium chloride to the buffer.

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Year:  1982        PMID： 7092140     DOI： 10.1177/000456328201900305

Source DB:  PubMed          Journal:  Ann Clin Biochem        ISSN： 0004-5632            Impact factor:   2.057


  13 in total

1.  Effect of L-arginine and L-lysine on lysosomal hydrolases and membrane bound phosphatases in experimentally induced myocardial infarction in rats.

Authors:  Kesavarao Kumar Ebenezar; Venkatachalem Sathish; Thiruvengadam Devaki
Journal:  Mol Cell Biochem       Date:  2003-05       Impact factor: 3.396

2.  Alterations in certain lysosomal glycohydrolases and cathepsins in rats on dexamethasone administration.

Authors:  S Rajashree; R Puvanakrishnan
Journal:  Mol Cell Biochem       Date:  1996-01-26       Impact factor: 3.396

3.  Antilipoperoxidative and membrane stabilizing effect of diosgenin, in experimentally induced myocardial infarction.

Authors:  K S Jayachandran; Hannah R Vasanthi; G V Rajamanickam
Journal:  Mol Cell Biochem       Date:  2009-02-21       Impact factor: 3.396

4.  Renal tubular dysfunction in alpha-thalassemia.

Authors:  Achra Sumboonnanonda; Prida Malasit; Voravarn S Tanphaichitr; Sompong Ong-ajyooth; Siripan Petrarat; Arun Vongjirad
Journal:  Pediatr Nephrol       Date:  2003-02-26       Impact factor: 3.714

5.  Modulation of cyclophosphamide-induced early lung injury by curcumin, an anti-inflammatory antioxidant.

Authors:  N Venkatesan; G Chandrakasan
Journal:  Mol Cell Biochem       Date:  1995-01-12       Impact factor: 3.396

6.  Cyclophosphamide induced early biochemical changes in lung lavage fluid and alterations in lavage cell function.

Authors:  N Venkatesan; G Chandrakasan
Journal:  Lung       Date:  1994       Impact factor: 2.584

7.  Metabolism of glycosaminoglycans in tissues of adjuvant arthritic rat.

Authors:  G K Reddy; S C Dhar
Journal:  Mol Cell Biochem       Date:  1991-08-14       Impact factor: 3.396

8.  UK Prospective Diabetes Study (UKPDS). VIII. Study design, progress and performance.

Authors: 
Journal:  Diabetologia       Date:  1991-12       Impact factor: 10.122

9.  Protective effect of Terminalia chebula against lysosomal enzyme alterations in isoproterenol-induced cardiac damage in rats.

Authors:  Subramaniyan Suchalatha; Chennam Srinivasulu Shyamala Devi
Journal:  Exp Clin Cardiol       Date:  2005

10.  Plasma N-acetyl-beta, D-glucosaminidase activities and glycaemia in diabetes mellitus.

Authors:  P Y Poon; T M Davis; T L Dornan; R C Turner
Journal:  Diabetologia       Date:  1983-06       Impact factor: 10.122
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