| Literature DB >> 19632350 |
Jana Avbersek1, Sandra Janezic, Mateja Pate, Maja Rupnik, Valerija Zidaric, Katarina Logar, Modest Vengust, Mateja Zemljic, Tina Pirs, Matjaz Ocepek.
Abstract
A study of Clostridium difficile diversity in pigs, calves and horses in Slovenia was conducted. A total of 547 samples were collected and C. difficile was isolated from 247/485 (50.9%) piglet samples, from 4/42 (9.5%) calf samples, and 1/20 (5%) horse samples. The isolates were characterized by toxinotyping, PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) using restriction endonuclease SmaI. Piglet isolates belonged to two toxinotypes (V and 0), four PCR-ribotypes (066, 029, SI 011, SI 010), and six pulsotypes. Bovine isolates were grouped into two toxinotypes (XIa and 0), three PCR-ribotypes (077, 002, 033), and three pulsotypes. The only equine isolate was indistinguishable from one calf isolate (XIa/033) in toxinotype, PCR-ribotype, and pulsotype. None of detected genotypes was present in all three animal hosts.Entities:
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Year: 2009 PMID： 19632350 DOI： 10.1016/j.anaerobe.2009.07.004
Source DB: PubMed Journal: Anaerobe ISSN： 1075-9964 Impact factor: 3.331