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Literature DB >> 1961715

GAL4 is phosphorylated as a consequence of transcriptional activation.

I Sadowski¹, D Niedbala, K Wood, M Ptashne.

Abstract

GAL4 protein isolated from yeast in which it is active is phosphorylated predominantly on two different serine residues. One of these was identified as Ser-837; substitution of this residue for alanine has no detectable effect on transcriptional activation by GAL4. Phosphorylation at Ser-837 requires that both the DNA binding and transcriptional activation functions be intact. We propose that some phosphorylations of GAL4, including that at Ser-837, occur concomitantly with activation of transcription.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1991 PMID： 1961715 PMCID： PMC52958 DOI： 10.1073/pnas.88.23.10510

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

1. Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation.

Authors: D Song; J W Dolan; Y L Yuan; S Fields
Journal: Genes Dev Date: 1991-05 Impact factor: 11.361

2. Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein.

Authors: L Keegan; G Gill; M Ptashne
Journal: Science Date: 1986-02-14 Impact factor: 47.728

3. Mutants of GAL4 protein altered in an activation function.

Authors: G Gill; M Ptashne
Journal: Cell Date: 1987-10-09 Impact factor: 41.582

4. Deletion analysis of GAL4 defines two transcriptional activating segments.

Authors: J Ma; M Ptashne
Journal: Cell Date: 1987-03-13 Impact factor: 41.582

5. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.

Authors: G M Rubin
Journal: J Biol Chem Date: 1973-06-10 Impact factor: 5.157

6. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.

Authors: L A Allison; M Moyle; M Shales; C J Ingles
Journal: Cell Date: 1985-09 Impact factor: 41.582

Review 7. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae.

Authors: M Johnston
Journal: Microbiol Rev Date: 1987-12

8. Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors: T A Kunkel
Journal: Proc Natl Acad Sci U S A Date: 1985-01 Impact factor: 11.205

9. A noncatalytic domain conserved among cytoplasmic protein-tyrosine kinases modifies the kinase function and transforming activity of Fujinami sarcoma virus P130gag-fps.

Authors: I Sadowski; J C Stone; T Pawson
Journal: Mol Cell Biol Date: 1986-12 Impact factor: 4.272

7. The regulator of the yeast proline utilization pathway is differentially phosphorylated in response to the quality of the nitrogen source.

Authors: H L Huang; M C Brandriss
Journal: Mol Cell Biol Date: 2000-02 Impact factor: 4.272

8. Multiple signals regulate GAL transcription in yeast.

Authors: J R Rohde; J Trinh; I Sadowski
Journal: Mol Cell Biol Date: 2000-06 Impact factor: 4.272

9. Multiple mechanisms provide rapid and stringent glucose repression of GAL gene expression in Saccharomyces cerevisiae.

Authors: M Johnston; J S Flick; T Pexton
Journal: Mol Cell Biol Date: 1994-06 Impact factor: 4.272

10. Zinc rapidly induces a metal response element-binding factor.

Authors: M Czupryn; W E Brown; B L Vallee
Journal: Proc Natl Acad Sci U S A Date: 1992-11-01 Impact factor: 11.205

GAL4 is phosphorylated as a consequence of transcriptional activation.

1. Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation.

2. Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein.

3. Mutants of GAL4 protein altered in an activation function.

4. Deletion analysis of GAL4 defines two transcriptional activating segments.

5. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.

6. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.

Review 7. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae.

8. Rapid and efficient site-specific mutagenesis without phenotypic selection.

9. A noncatalytic domain conserved among cytoplasmic protein-tyrosine kinases modifies the kinase function and transforming activity of Fujinami sarcoma virus P130gag-fps.

10. Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein.

1. Transcriptional activating regions target a cyclin-dependent kinase.

2. Regions of GAL4 critical for binding to a promoter in vivo revealed by a visual DNA-binding analysis.

3. A transcriptionally active form of GAL4 is phosphorylated and associated with GAL80.

4. A two-hybrid system for transactivator bait proteins.

5. A genetic system for studying the activity of a proteolytic enzyme.

6. Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms.

7. The regulator of the yeast proline utilization pathway is differentially phosphorylated in response to the quality of the nitrogen source.

8. Multiple signals regulate GAL transcription in yeast.

9. Multiple mechanisms provide rapid and stringent glucose repression of GAL gene expression in Saccharomyces cerevisiae.

10. Zinc rapidly induces a metal response element-binding factor.