Literature DB >> 195982

Solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus antibody.

L A Babiuk, S D Acres, B T Rouse.   

Abstract

An indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. The test involved ethanol fixation of microcultures of bovine rotavirus-infected BSC-1 cells and reacetion with bovine antirotavirus serum, followed by 125I-labeled rabbit anti-bovine immunoglobulin G. The technique was shown to be virus specific and highly sensitive. The fixed microcultures could be stored at 4 degrees C for at least 2 months without affecting the sensitivity of the test. The application of this system for the detection of rotavirus antibodies in humans is briefly discussed.

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Year:  1977        PMID： 195982      PMCID： PMC274688          DOI： 10.1128/jcm.6.1.10-15.1977

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

Review 1.  Viral enteritis of calves.

Authors:  G N Woode; J C Bridger
Journal:  Vet Rec       Date:  1975-01-25       Impact factor: 2.695

2.  Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody.

Authors:  P J Middleton; M Petric; C M Hewitt; M T Szymanski; J S Tam
Journal:  J Clin Pathol       Date:  1976-03       Impact factor: 3.411

3.  Relation between viruses from acute gastroenteritis of children and newborn calves.

Authors:  T H Flewett; A S Bryden; H Davies; G N Woode; J C Bridger; J M Derrick
Journal:  Lancet       Date:  1974-07-13       Impact factor: 79.321

4.  Labeled staphylococcal protein A as an immunological probe in the analysis of cell surface markers.

Authors:  G Dorval; K I Welsh; H Wigzell
Journal:  Scand J Immunol       Date:  1974       Impact factor: 3.487

5.  Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis.

Authors:  R F Bishop; G P Davidson; I H Holmes; B J Ruck
Journal:  Lancet       Date:  1973-12-08       Impact factor: 79.321

6.  Cell culture adaptation and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in Nebraska.

Authors:  A L Fernelius; A E Ritchie; L G Classick; J O Norman; C A Mebus
Journal:  Arch Gesamte Virusforsch       Date:  1972

7.  Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125 I-antibody.

Authors:  C M Ling; L R Overby
Journal:  J Immunol       Date:  1972-10       Impact factor: 5.422

8.  Detection by radioimmunoassay of antibodies in human smallpox patients and vaccinees.

Authors:  D W Ziegler; H D Hutchinson; J P Koplan; J H Nakano
Journal:  J Clin Microbiol       Date:  1975-03       Impact factor: 5.948

9.  Solid phase radioimmunoassay for typing herpes simplex viral antibodies in human sera.

Authors:  B Forghani; N J Schmidt; E H Lennette
Journal:  J Clin Microbiol       Date:  1975-11       Impact factor: 5.948

10.  Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test.

Authors:  M W Peterson; R S Spendlove; R A Smart
Journal:  J Clin Microbiol       Date:  1976-03       Impact factor: 5.948
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  9 in total

1.  Comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey.

Authors:  L H Ghose; R D Schnagl; I H Holmes
Journal:  J Clin Microbiol       Date:  1978-09       Impact factor: 5.948

2.  Rotavirus isolation and cultivation in the presence of trypsin.

Authors:  L A Babiuk; K Mohammed; L Spence; M Fauvel; R Petro
Journal:  J Clin Microbiol       Date:  1977-12       Impact factor: 5.948

3.  Sensitive solid-phase radioimmunoassay for detection of human immunoglobulin G antibodies to varicella-zoster virus.

Authors:  M G Friedman; S Leventon-Kriss; I Sarov
Journal:  J Clin Microbiol       Date:  1979-01       Impact factor: 5.948

4.  Genetic heterogeneity within individual bovine rotavirus isolates.

Authors:  M Sabara; D Deregt; L A Babiuk; V Misra
Journal:  J Virol       Date:  1982-12       Impact factor: 5.103

5.  Immunization of calves against enterotoxigenic colibacillosis by vaccinating dams with purified K99 antigen and whole cell bacterins.

Authors:  S D Acres; R E Isaacson; L A Babiuk; R A Kapitany
Journal:  Infect Immun       Date:  1979-07       Impact factor: 3.441

6.  Effect of tunicamycin on rotavirus assembly and infectivity.

Authors:  M Sabara; L A Babiuk; J Gilchrist; V Misra
Journal:  J Virol       Date:  1982-09       Impact factor: 5.103

7.  A longitudinal cohort study in calves evaluated for rotavirus infections from 1 to 12 months of age by sequential serological assays.

Authors:  Dianjun Cao; Blessing Igboeli; Lijuan Yuan; Albert Z Kapikian; Jess L Ayers; Francis R Abinanti; Yasutaka Hoshino
Journal:  Arch Virol       Date:  2009-04-03       Impact factor: 2.574

8.  Immune mechanisms against canine distemper. III. Role of complement lysis in the immunity and persistent infection of canine distemper virus.

Authors:  C K Ho; L A Babiuk
Journal:  Immunology       Date:  1980-02       Impact factor: 7.397

Review 9.  Rotaviral and coronaviral diarrhea.

Authors:  A Torres-Medina; D H Schlafer; C A Mebus
Journal:  Vet Clin North Am Food Anim Pract       Date:  1985-11       Impact factor: 3.357
  9 in total

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