Literature DB >> 6292451

Effect of tunicamycin on rotavirus assembly and infectivity.

M Sabara, L A Babiuk, J Gilchrist, V Misra.   

Abstract

Bovine rotavirus grown in the presence or absence of tunicamycin was analyzed with respect to yield of infectious virus, the ratio of complete to incomplete particles, and polypeptide composition. Tunicamycin at a concentration of 1 microgram/ml reduced virus yields by 4 logs and completely prevented the incorporation of [3H]uridine into complete rotavirus particles, as determined by cesium chloride gradient analysis. Concomitant with a reduction in complete particles, three rotavirus polypeptides shifted in their relative position on polyacrylamide gels from 41,900-molecular-weight position (41.9K), 29.3K, and 16.1K to migrate at 35.5K, 22.7K, and 15.5K, respectively. Limited proteolysis indicated that the lower-molecular-weight polypeptides possessed the same constituent peptides as the larger polypeptides, suggesting that they represented the unglycosylated equivalents. These results suggest that interference with glycosylation prevents proper assembly of the outer coat proteins in bovine rotavirus.

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Year:  1982        PMID： 6292451      PMCID： PMC256220     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  29 in total

1.  Characterisation of a rotavirus.20b.

Authors:  J F Newman; F Brown; J C Bridger; G N Woode
Journal:  Nature       Date:  1975-12-18       Impact factor: 49.962

2.  Studies on the assembly of the envelope of Newcastle disease virus.

Authors:  Y Nagai; H Ogura; H Klenk
Journal:  Virology       Date:  1976-02       Impact factor: 3.616

3.  Characterization of two particle types of calf rotavirus.

Authors:  J C Bridger; G N Woode
Journal:  J Gen Virol       Date:  1976-05       Impact factor: 3.891

4.  Evidence for a glycoprotein in reovirus.

Authors:  G Krystal; J Perrault; A F Graham
Journal:  Virology       Date:  1976-07-15       Impact factor: 3.616

5.  The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody.

Authors:  J M Kelley; S U Emerson; R R Wagner
Journal:  J Virol       Date:  1972-12       Impact factor: 5.103

6.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Location of the glycoprotein in the membrane of Sindbis virus.

Authors:  R W Compans
Journal:  Nat New Biol       Date:  1971-01-27

9.  Replication of Sindbis virus. IV. Electron microscope study of the insertion of viral glycoproteins into the surface of infected chick cells.

Authors:  C R Birdwell; J H Strauss
Journal:  J Virol       Date:  1974-08       Impact factor: 5.103

10.  Suppression of glycoprotein formation of Semliki Forest, influenza, and avian sarcoma virus by tunicamycin.

Authors:  R T Schwarz; J M Rohrschneider; M F Schmidt
Journal:  J Virol       Date:  1976-09       Impact factor: 5.103
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  18 in total

1.  Receptor activity of rotavirus nonstructural glycoprotein NS28.

Authors:  K S Au; W K Chan; J W Burns; M K Estes
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

2.  Dissecting rotavirus particle-raft interaction with small interfering RNAs: insights into rotavirus transit through the secretory pathway.

Authors:  Mariela A Cuadras; Bruno B Bordier; Jose L Zambrano; Juan E Ludert; Harry B Greenberg
Journal:  J Virol       Date:  2006-04       Impact factor: 5.103

3.  Rotavirus protein rearrangements in purified membrane-enveloped intermediate particles.

Authors:  M S Poruchynsky; P H Atkinson
Journal:  J Virol       Date:  1991-09       Impact factor: 5.103

4.  Carbohydrates facilitate correct disulfide bond formation and folding of rotavirus VP7.

Authors:  A Mirazimi; L Svensson
Journal:  J Virol       Date:  1998-05       Impact factor: 5.103

5.  The cytoplasmic tail of NSP4, the endoplasmic reticulum-localized non-structural glycoprotein of rotavirus, contains distinct virus binding and coiled coil domains.

Authors:  J A Taylor; J A O'Brien; M Yeager
Journal:  EMBO J       Date:  1996-09-02       Impact factor: 11.598

Review 6.  Rotavirus gene structure and function.

Authors:  M K Estes; J Cohen
Journal:  Microbiol Rev       Date:  1989-12

7.  Preliminary characterization of an epitope involved in neutralization and cell attachment that is located on the major bovine rotavirus glycoprotein.

Authors:  M Sabara; J E Gilchrist; G R Hudson; L A Babiuk
Journal:  J Virol       Date:  1985-01       Impact factor: 5.103

8.  Intracellular localization of rotaviral proteins.

Authors:  S C Richardson; L E Mercer; S Sonza; I H Holmes
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

9.  Identification of a bovine rotavirus gene and gene product influencing cellular attachment.

Authors:  M Sabara; L A Babiuk
Journal:  J Virol       Date:  1984-08       Impact factor: 5.103

10.  Spike protein VP4 assembly with maturing rotavirus requires a postendoplasmic reticulum event in polarized caco-2 cells.

Authors:  Olivier Delmas; Anne-Marie Durand-Schneider; Jean Cohen; Odile Colard; Germain Trugnan
Journal:  J Virol       Date:  2004-10       Impact factor: 5.103
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