| Literature DB >> 19575250 |
Umar Masood Quraishi1, Michael Abrouk, Stéphanie Bolot, Caroline Pont, Mickael Throude, Nicolas Guilhot, Carole Confolent, Fernanda Bortolini, Sébastien Praud, Alain Murigneux, Gilles Charmet, Jerome Salse.
Abstract
Recent updates in comparative genomics among cereals have provided the opportunity to identify conserved orthologous set (COS) DNA sequences for cross-genome map-based cloning of candidate genes underpinning quantitative traits. New tools are described that are applicable to any cereal genome of interest, namely, alignment criterion for orthologous couples identification, as well as the Intron Spanning Marker software to automatically select intron-spanning primer pairs. In order to test the software, it was applied to the bread wheat genome, and 695 COS markers were assigned to 1,535 wheat loci (on average one marker/2.6 cM) based on 827 robust rice-wheat orthologs. Furthermore, 31 of the 695 COS markers were selected to fine map a pentosan viscosity quantitative trait loci (QTL) on wheat chromosome 7A. Among the 31 COS markers, 14 (45%) were polymorphic between the parental lines and 12 were mapped within the QTL confidence interval with one marker every 0.6 cM defining candidate genes among the rice orthologous region.Entities:
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Year: 2009 PMID： 19575250 DOI： 10.1007/s10142-009-0129-8
Source DB: PubMed Journal: Funct Integr Genomics ISSN： 1438-793X Impact factor: 3.410