| Literature DB >> 15514046 |
L L Qi1, B Echalier, S Chao, G R Lazo, G E Butler, O D Anderson, E D Akhunov, J Dvorák, A M Linkiewicz, A Ratnasiri, J Dubcovsky, C E Bermudez-Kandianis, R A Greene, R Kantety, C M La Rota, J D Munkvold, S F Sorrells, M E Sorrells, M Dilbirligi, D Sidhu, M Erayman, H S Randhawa, D Sandhu, S N Bondareva, K S Gill, A A Mahmoud, X-F Ma, J P Gustafson, E J Conley, V Nduati, J L Gonzalez-Hernandez, J A Anderson, J H Peng, N L V Lapitan, K G Hossain, V Kalavacharla, S F Kianian, M S Pathan, D S Zhang, H T Nguyen, D-W Choi, R D Fenton, T J Close, P E McGuire, C O Qualset, B S Gill.
Abstract
Because of the huge size of the common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) genome of 17,300 Mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. Each EST detected a mean of 4.8 restriction fragments and 2.8 loci. More loci were mapped in the B genome (5774) than in the A (5173) or D (5146) genomes. The EST density was significantly higher for the D genome than for the A or B. In general, EST density increased relative to the physical distance from the centromere. The majority of EST-dense regions are in the distal parts of chromosomes. Most of the agronomically important genes are located in EST-dense regions. The chromosome bin map of ESTs is a unique resource for SNP analysis, comparative mapping, structural and functional analysis, and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome.Entities:
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Year: 2004 PMID： 15514046 PMCID： PMC1448828 DOI： 10.1534/genetics.104.034868
Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562