OBJECTIVES: To examine the degree of conversion (DC) of the adhesive interfaces created by Filtek Silorane Adhesive and Clearfil SE Bond using micro-Raman spectroscopy. METHODS: The adhesives were applied on human dentin in accordance with manufacturer's instructions. Specimens were cut to expose the bonded interfaces to the micro-Raman beam (Ranishaw InVia; laser wl 785 nm). Raman spectra were collected along the dentin/self-etching primer/adhesive interface at 1 microm intervals. The relative intensities of bands associated with mineral (P-O functional group at 960 cm(-1)) and adhesive (C-C-O group at 605 cm(-1)) components within the bonded interface were used to detect monomer penetration into the dentin matrix and to calculate the degree of conversion (C=C at 1640 cm(-1) as reaction peak, C-C-O at 605 cm(-1) as reference peak). Data were statistically analyzed with two-way ANOVA. RESULTS: DC of Filtek Silorane Adhesive was 69+/-7% in the adhesive layer, increasing (p<0.05) to 93+/-5% in the primer and 92+/-9% in the hybrid layer. Clearfil SE Bond showed a DC of 83+/-3% in the hybrid and 85+/-3% in the adhesive layer. Thus, Filtek Silorane Adhesive showed a higher DC than Clearfil SE Bond in the hybrid layer (p<0.05), but a lower DC in the adhesive (p<0.05). SIGNIFICANCE: As high DC is a fundamental pre-requisite for the stability of the bond over time, this study supports the hypothesis that optimal stability of Filtek Silorane Adhesive can be obtained. However, further research is needed to investigate the mechanical properties of the hybrid layer created by Filtek Silorane Adhesive and its long-term stability.
OBJECTIVES: To examine the degree of conversion (DC) of the adhesive interfaces created by Filtek Silorane Adhesive and Clearfil SE Bond using micro-Raman spectroscopy. METHODS: The adhesives were applied on human dentin in accordance with manufacturer's instructions. Specimens were cut to expose the bonded interfaces to the micro-Raman beam (Ranishaw InVia; laser wl 785 nm). Raman spectra were collected along the dentin/self-etching primer/adhesive interface at 1 microm intervals. The relative intensities of bands associated with mineral (P-O functional group at 960 cm(-1)) and adhesive (C-C-O group at 605 cm(-1)) components within the bonded interface were used to detect monomer penetration into the dentin matrix and to calculate the degree of conversion (C=C at 1640 cm(-1) as reaction peak, C-C-O at 605 cm(-1) as reference peak). Data were statistically analyzed with two-way ANOVA. RESULTS:DC of Filtek Silorane Adhesive was 69+/-7% in the adhesive layer, increasing (p<0.05) to 93+/-5% in the primer and 92+/-9% in the hybrid layer. Clearfil SE Bond showed a DC of 83+/-3% in the hybrid and 85+/-3% in the adhesive layer. Thus, Filtek Silorane Adhesive showed a higher DC than Clearfil SE Bond in the hybrid layer (p<0.05), but a lower DC in the adhesive (p<0.05). SIGNIFICANCE: As high DC is a fundamental pre-requisite for the stability of the bond over time, this study supports the hypothesis that optimal stability of Filtek Silorane Adhesive can be obtained. However, further research is needed to investigate the mechanical properties of the hybrid layer created by Filtek Silorane Adhesive and its long-term stability.
Authors: Tamires T Maske; Cristina P Isolan; Françoise H van de Sande; Aline C Peixoto; André L Faria-E-Silva; Maximiliano S Cenci; Rafael R Moraes Journal: Clin Oral Investig Date: 2014-10-17 Impact factor: 3.573
Authors: Renata Kirita Doi Sampaio; Linda Wang; Rodrigo Varella de Carvalho; Eugenio José Garcia; Andréa Mello de Andrade; Celso Afonso Klein-Júnior; Rosa Helena Miranda Grande; Sandra Kiss Moura Journal: J Appl Oral Sci Date: 2013 Jan-Feb Impact factor: 2.698
Authors: Yasmine M Pupo; Paulo Vitor Farago; Jessica M Nadal; Luzia C Simão; Luís Antônio Esmerino; Osnara M M Gomes; João Carlos Gomes Journal: Int J Mol Sci Date: 2014-05-20 Impact factor: 5.923
Authors: Mehdi Abed Kahnemooyi; Amir Ahmad Ajami; Soodabeh Kimyai; Fatemeh Pournaghiazar; Siavash Savadi Oskoee; Mohammad Ali Mhammadi Torkani Journal: J Dent Res Dent Clin Dent Prospects Date: 2014-12-03