OBJECTIVES: This study was designed to adapt a previously developed in vitro microcosm biofilm model to create carries-affected dentin (CAD) and establish conditions for using the model in bonding studies. MATERIALS AND METHODS: Biofilms were originated from human saliva and grown on dentin discs for 0 (sound dentin), 3, 5, 7, 14, or 21 days under intermittent cariogenic condition (n = 10). At each time point, composite cylinders were bonded to the dentin using self-etch adhesive (Clearfil SE Bond). The response variables were integrated mineral loss (ΔS), lesion depth (LD), shear bond strength (SBS), and failure mode. Data were statistically analyzed (α = 0.05). Bonded interfaces were analyzed by scanning electron microscopy (SEM), and dentin surfaces characterized by infrared spectroscopy (Fourier transform infrared spectroscopy, FTIR). RESULTS: Lower ΔS was found for sound dentin than for CAD in all experimental groups, except for the group under cariogenic challenge for 3 days. The SBS to CAD was significantly lower than control for all cariogenic challenge times. Adhesive failures were predominant in all groups. ΔS and LD had a significant negative correlation with SBS. A significant exponential decay in SBS was associated with increased ΔS values. CAD had lower mineral and amide I content and an irregular hybridization interface compared to sound dentin. CONCLUSIONS: The microcosm biofilm model was able to artificially induce CAD, which imposed challenge to the bonding of the polymeric adhesive material. CLINICAL RELEVANCE: Presence of CAD might interfere with the bonding of polymeric materials. The microcosm biofilm model proposed could be useful for preclinical dentin bonding studies.
OBJECTIVES: This study was designed to adapt a previously developed in vitro microcosm biofilm model to create carries-affected dentin (CAD) and establish conditions for using the model in bonding studies. MATERIALS AND METHODS: Biofilms were originated from human saliva and grown on dentin discs for 0 (sound dentin), 3, 5, 7, 14, or 21 days under intermittent cariogenic condition (n = 10). At each time point, composite cylinders were bonded to the dentin using self-etch adhesive (Clearfil SE Bond). The response variables were integrated mineral loss (ΔS), lesion depth (LD), shear bond strength (SBS), and failure mode. Data were statistically analyzed (α = 0.05). Bonded interfaces were analyzed by scanning electron microscopy (SEM), and dentin surfaces characterized by infrared spectroscopy (Fourier transform infrared spectroscopy, FTIR). RESULTS: Lower ΔS was found for sound dentin than for CAD in all experimental groups, except for the group under cariogenic challenge for 3 days. The SBS to CAD was significantly lower than control for all cariogenic challenge times. Adhesive failures were predominant in all groups. ΔS and LD had a significant negative correlation with SBS. A significant exponential decay in SBS was associated with increased ΔS values. CAD had lower mineral and amide I content and an irregular hybridization interface compared to sound dentin. CONCLUSIONS: The microcosm biofilm model was able to artificially induce CAD, which imposed challenge to the bonding of the polymeric adhesive material. CLINICAL RELEVANCE: Presence of CAD might interfere with the bonding of polymeric materials. The microcosm biofilm model proposed could be useful for preclinical dentin bonding studies.
Authors: M Yoshiyama; F R Tay; J Doi; Y Nishitani; T Yamada; K Itou; R M Carvalho; M Nakajima; D H Pashley Journal: J Dent Res Date: 2002-08 Impact factor: 6.116
Authors: Chiara O Navarra; Milena Cadenaro; Steven R Armstrong; Julie Jessop; Francesca Antoniolli; Valter Sergo; Roberto Di Lenarda; Lorenzo Breschi Journal: Dent Mater Date: 2009-06-30 Impact factor: 5.304
Authors: Egija Zaura; Mark J Buijs; Michel A Hoogenkamp; Lena Ciric; Adele Papetti; Caterina Signoretto; Monica Stauder; Peter Lingström; Jonathan Pratten; David A Spratt; Michael Wilson Journal: J Biomed Biotechnol Date: 2011-09-14