Literature DB >> 19566682

Tryptophan auxotrophs were obtained by random transposon insertions in the Methanococcus maripaludis tryptophan operon.

Iris Porat, William B Whitman.   

Abstract

Methanococcus maripaludis is an anaerobic, methane-producing archaeon that utilizes H(2) or formate for the reduction of CO(2) to methane. Tryptophan auxotrophs were constructed by in vitro insertions of the Tn5 transposon into the tryptophan operon, followed by transformation into M. maripaludis. This method could serve for rapid insertions into large cloned DNA regions.

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Year:  2009        PMID: 19566682      PMCID: PMC2771393          DOI: 10.1111/j.1574-6968.2009.01689.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  18 in total

1.  Role of Amino Acids and Vitamins in Nutrition of Mesophilic Methanococcus spp.

Authors:  W B Whitman; S Sohn; S Kuk; R Xing
Journal:  Appl Environ Microbiol       Date:  1987-10       Impact factor: 4.792

2.  Growth and plating efficiency of methanococci on agar media.

Authors:  W J Jones; W B Whitman; R D Fields; R S Wolfe
Journal:  Appl Environ Microbiol       Date:  1983-07       Impact factor: 4.792

3.  Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis.

Authors:  Jun Kai Zhang; Andrea K White; H Craig Kuettner; Paolo Boccazzi; William W Metcalf
Journal:  J Bacteriol       Date:  2002-03       Impact factor: 3.490

4.  Bacterial taxa that limit sulfur flux from the ocean.

Authors:  Erinn C Howard; James R Henriksen; Alison Buchan; Chris R Reisch; Helmut Bürgmann; Rory Welsh; Wenying Ye; José M González; Kimberly Mace; Samantha B Joye; Ronald P Kiene; William B Whitman; Mary Ann Moran
Journal:  Science       Date:  2006-10-27       Impact factor: 47.728

5.  Expression vectors for Methanococcus maripaludis: overexpression of acetohydroxyacid synthase and beta-galactosidase.

Authors:  W L Gardner; W B Whitman
Journal:  Genetics       Date:  1999-08       Impact factor: 4.562

6.  Isolation of acetate auxotrophs of the methane-producing archaeon Methanococcus maripaludis by random insertional mutagenesis.

Authors:  W Kim; W B Whitman
Journal:  Genetics       Date:  1999-08       Impact factor: 4.562

7.  Method for isolation of auxotrophs in the methanogenic archaebacteria: role of the acetyl-CoA pathway of autotrophic CO2 fixation in Methanococcus maripaludis.

Authors:  J Ladapo; W B Whitman
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

8.  Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis.

Authors:  E L Hendrickson; R Kaul; Y Zhou; D Bovee; P Chapman; J Chung; E Conway de Macario; J A Dodsworth; W Gillett; D E Graham; M Hackett; A K Haydock; A Kang; M L Land; R Levy; T J Lie; T A Major; B C Moore; I Porat; A Palmeiri; G Rouse; C Saenphimmachak; D Söll; S Van Dien; T Wang; W B Whitman; Q Xia; Y Zhang; F W Larimer; M V Olson; J A Leigh
Journal:  J Bacteriol       Date:  2004-10       Impact factor: 3.490

9.  Pathway of acetate assimilation in autotrophic and heterotrophic methanococci.

Authors:  J S Shieh; W B Whitman
Journal:  J Bacteriol       Date:  1987-11       Impact factor: 3.490

10.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

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  5 in total

1.  Genome-scale analysis of gene function in the hydrogenotrophic methanogenic archaeon Methanococcus maripaludis.

Authors:  Felipe Sarmiento; Jan Mrázek; William B Whitman
Journal:  Proc Natl Acad Sci U S A       Date:  2013-03-04       Impact factor: 11.205

2.  Transposon Insertion Mutagenesis in Hyperthermophilic Crenarchaeon Sulfolobus islandicus.

Authors:  Changyi Zhang; Rachel J Whitaker
Journal:  Methods Mol Biol       Date:  2022

3.  Genetic confirmation of the role of sulfopyruvate decarboxylase in coenzyme M biosynthesis in Methanococcus maripaludis.

Authors:  Felipe Sarmiento; Courtney K Ellison; William B Whitman
Journal:  Archaea       Date:  2013-09-12       Impact factor: 3.273

Review 4.  Methanogens: biochemical background and biotechnological applications.

Authors:  Franziska Enzmann; Florian Mayer; Michael Rother; Dirk Holtmann
Journal:  AMB Express       Date:  2018-01-04       Impact factor: 3.298

5.  A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens.

Authors:  Feng Long; Liangliang Wang; Boguslaw Lupa; William B Whitman
Journal:  Archaea       Date:  2017-11-19       Impact factor: 3.273

  5 in total

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