Literature DB >> 11844777

Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis.

Jun Kai Zhang1, Andrea K White, H Craig Kuettner, Paolo Boccazzi, William W Metcalf.   

Abstract

We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of proline auxotrophs with mutations in the proABC locus. Each mutation was also complemented in trans with autonomously replicating Methanosarcina-Escherichia plasmid shuttle vectors.

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Year:  2002        PMID： 11844777      PMCID： PMC134853          DOI： 10.1128/JB.184.5.1449-1454.2002

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

1.  A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites.

Authors:  E Frengen; D Weichenhan; B Zhao; K Osoegawa; M van Geel; P J de Jong
Journal:  Genomics       Date:  1999-06-15       Impact factor: 5.736

2.  Double cos site vectors: simplified cosmid cloning.

Authors:  P Bates
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

3.  Culture medium for enterobacteria.

Authors:  F C Neidhardt; P L Bloch; D F Smith
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

4.  Salmonella typhimurium proline mutants.

Authors:  H Itikawa; M Demerec
Journal:  J Bacteriol       Date:  1968-03       Impact factor: 3.490

5.  Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12.

Authors:  B L Wanner
Journal:  J Mol Biol       Date:  1986-09-05       Impact factor: 5.469

6.  Methanococcus jannaschii generates L-proline by cyclization of L-ornithine.

Authors:  M Graupner; R H White
Journal:  J Bacteriol       Date:  2001-09       Impact factor: 3.490

7.  Generation of dominant selectable markers for resistance to pseudomonic acid by cloning and mutagenesis of the ileS gene from the archaeon Methanosarcina barkeri fusaro.

Authors:  P Boccazzi; J K Zhang; W W Metcalf
Journal:  J Bacteriol       Date:  2000-05       Impact factor: 3.490

8.  Bacterial alkaline phosphatase clonal variation in some Escherichia coli K-12 phoR mutant strains.

Authors:  B L Wanner
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

9.  Structure of genes and an insertion element in the methane producing archaebacterium Methanobrevibacter smithii.

Authors:  P T Hamilton; J N Reeve
Journal:  Mol Gen Genet       Date:  1985

10.  Cosmid cloning and transposon mutagenesis in Salmonella typhimurium using phage lambda vehicles.

Authors:  E T Palva; P Liljeström; S Harayama
Journal:  Mol Gen Genet       Date:  1981
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  24 in total

1.  The genome of M. acetivorans reveals extensive metabolic and physiological diversity.

Authors:  James E Galagan; Chad Nusbaum; Alice Roy; Matthew G Endrizzi; Pendexter Macdonald; Will FitzHugh; Sarah Calvo; Reinhard Engels; Serge Smirnov; Deven Atnoor; Adam Brown; Nicole Allen; Jerome Naylor; Nicole Stange-Thomann; Kurt DeArellano; Robin Johnson; Lauren Linton; Paul McEwan; Kevin McKernan; Jessica Talamas; Andrea Tirrell; Wenjuan Ye; Andrew Zimmer; Robert D Barber; Isaac Cann; David E Graham; David A Grahame; Adam M Guss; Reiner Hedderich; Cheryl Ingram-Smith; H Craig Kuettner; Joseph A Krzycki; John A Leigh; Weixi Li; Jinfeng Liu; Biswarup Mukhopadhyay; John N Reeve; Kerry Smith; Timothy A Springer; Lowell A Umayam; Owen White; Robert H White; Everly Conway de Macario; James G Ferry; Ken F Jarrell; Hua Jing; Alberto J L Macario; Ian Paulsen; Matthew Pritchett; Kevin R Sowers; Ronald V Swanson; Steven H Zinder; Eric Lander; William W Metcalf; Bruce Birren
Journal:  Genome Res       Date:  2002-04       Impact factor: 9.043

2.  Development of a plasmid-mediated reporter system for in vivo monitoring of gene expression in the archaeon Methanosarcina acetivorans.

Authors:  Ethel E Apolinario; Karin M Jackson; Kevin R Sowers
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

3.  The archaeon Methanosarcina acetivorans contains a protein disulfide reductase with an iron-sulfur cluster.

Authors:  Daniel J Lessner; James G Ferry
Journal:  J Bacteriol       Date:  2007-08-03       Impact factor: 3.490

4.  Molecular cloning, sequence analysis, and heterologous expression of the phosphinothricin tripeptide biosynthetic gene cluster from Streptomyces viridochromogenes DSM 40736.

Authors:  Joshua A V Blodgett; Jun Kai Zhang; William W Metcalf
Journal:  Antimicrob Agents Chemother       Date:  2005-01       Impact factor: 5.191

5.  New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species.

Authors:  Adam M Guss; Michael Rother; Jun Kai Zhang; Gargi Kulkarni; William W Metcalf
Journal:  Archaea       Date:  2008-12       Impact factor: 3.273

6.  MrpA functions in energy conversion during acetate-dependent growth of Methanosarcina acetivorans.

Authors:  Ricardo Jasso-Chávez; Ethel E Apolinario; Kevin R Sowers; James G Ferry
Journal:  J Bacteriol       Date:  2013-07-08       Impact factor: 3.490

7.  Tryptophan auxotrophs were obtained by random transposon insertions in the Methanococcus maripaludis tryptophan operon.

Authors:  Iris Porat; William B Whitman
Journal:  FEMS Microbiol Lett       Date:  2009-06-12       Impact factor: 2.742

8.  Genome-scale metabolic reconstruction and hypothesis testing in the methanogenic archaeon Methanosarcina acetivorans C2A.

Authors:  Matthew N Benedict; Matthew C Gonnerman; William W Metcalf; Nathan D Price
Journal:  J Bacteriol       Date:  2011-12-02       Impact factor: 3.490

9.  Methanol-dependent gene expression demonstrates that methyl-coenzyme M reductase is essential in Methanosarcina acetivorans C2A and allows isolation of mutants with defects in regulation of the methanol utilization pathway.

Authors:  Michael Rother; Paolo Boccazzi; Arpita Bose; Matthew A Pritchett; W W Metcalf
Journal:  J Bacteriol       Date:  2005-08       Impact factor: 3.490

10.  Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea.

Authors:  Matthew A Pritchett; Jun Kai Zhang; William W Metcalf
Journal:  Appl Environ Microbiol       Date:  2004-03       Impact factor: 4.792
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