Literature DB >> 19525960

Quantitative analysis of gene expression in a single cell by qPCR.

Kiyomi Taniguchi1, Tomoharu Kajiyama, Hideki Kambara.   

Abstract

We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or less. This method is sufficiently accurate to investigate the heterogeneity of single cells.

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Year:  2009        PMID： 19525960     DOI： 10.1038/nmeth.1338

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  19 in total

Review 1.  Gene expression and the myth of the average cell.

Authors:  Jeffrey M Levsky; Robert H Singer
Journal:  Trends Cell Biol       Date:  2003-01       Impact factor: 20.808

2.  Quantitative measurement of mRNA at different loci within an individual living cell.

Authors:  Hironori Uehara; Toshiya Osada; Atsushi Ikai
Journal:  Ultramicroscopy       Date:  2004-08       Impact factor: 2.689

3.  Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in the portal vein area.

Authors:  Kazuhiko Tsuji; Kensuke Yamauchi; Meng Yang; Ping Jiang; Michael Bouvet; Hitoshi Endo; Yoshikatsu Kanai; Koji Yamashita; Abdool R Moossa; Robert M Hoffman
Journal:  Cancer Res       Date:  2006-01-01       Impact factor: 12.701

4.  Parallel picoliter rt-PCR assays using microfluidics.

Authors:  Joshua S Marcus; W French Anderson; Stephen R Quake
Journal:  Anal Chem       Date:  2006-02-01       Impact factor: 6.986

5.  Solid-phase cDNA library construction, a versatile approach.

Authors:  T Roeder
Journal:  Nucleic Acids Res       Date:  1998-07-15       Impact factor: 16.971

6.  Amplification of mRNA populations using aRNA generated from immobilized oligo(dT)-T7 primed cDNA.

Authors:  J Eberwine
Journal:  Biotechniques       Date:  1996-04       Impact factor: 1.993

7.  Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real-time RT-PCR.

Authors:  Akiko Wagatsuma; Hisayo Sadamoto; Takashi Kitahashi; Ken Lukowiak; Akihisa Urano; Etsuro Ito
Journal:  J Exp Biol       Date:  2005-06       Impact factor: 3.312

8.  Single cell resolution in regulation of gene expression.

Authors:  Orli G Bahcall
Journal:  Mol Syst Biol       Date:  2005-06-28       Impact factor: 11.429

Review 9.  Dynamics of single-cell gene expression.

Authors:  Diane Longo; Jeff Hasty
Journal:  Mol Syst Biol       Date:  2006-11-28       Impact factor: 11.429

10.  An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis.

Authors:  Kazuki Kurimoto; Yukihiro Yabuta; Yasuhide Ohinata; Yukiko Ono; Kenichiro D Uno; Rikuhiro G Yamada; Hiroki R Ueda; Mitinori Saitou
Journal:  Nucleic Acids Res       Date:  2006-03-17       Impact factor: 16.971
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  53 in total

Review 1.  Single cell analysis: the new frontier in 'omics'.

Authors:  Daojing Wang; Steven Bodovitz
Journal:  Trends Biotechnol       Date:  2010-04-29       Impact factor: 19.536

2.  The effect of acute dose charge particle radiation on expression of DNA repair genes in mice.

Authors:  Muhammad Akram Tariq; Ayodotun Soedipe; Govindarajan Ramesh; Honglu Wu; Ye Zhang; Shishir Shishodia; Daila S Gridley; Nader Pourmand; Olufisayo Jejelowo
Journal:  Mol Cell Biochem       Date:  2010-11-16       Impact factor: 3.396

3.  Probes for intracellular RNA imaging in live cells.

Authors:  Philip J Santangelo; Eric Alonas; Jeenah Jung; Aaron W Lifland; Chiara Zurla
Journal:  Methods Enzymol       Date:  2012       Impact factor: 1.600

4.  Central role of the cell in microbial ecology.

Authors:  Karsten Zengler
Journal:  Microbiol Mol Biol Rev       Date:  2009-12       Impact factor: 11.056

5.  Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq.

Authors:  Saiful Islam; Una Kjällquist; Annalena Moliner; Pawel Zajac; Jian-Bing Fan; Peter Lönnerberg; Sten Linnarsson
Journal:  Genome Res       Date:  2011-05-04       Impact factor: 9.043

6.  Digital transcriptome profiling from attomole-level RNA samples.

Authors:  Fatih Ozsolak; Alon Goren; Melissa Gymrek; Mitchell Guttman; Aviv Regev; Bradley E Bernstein; Patrice M Milos
Journal:  Genome Res       Date:  2010-02-04       Impact factor: 9.043

7.  Analysis of gene expression at the single-cell level using microdroplet-based microfluidic technology.

Authors:  Pascaline Mary; Luce Dauphinot; Nadège Bois; Marie-Claude Potier; Vincent Studer; Patrick Tabeling
Journal:  Biomicrofluidics       Date:  2011-06-03       Impact factor: 2.800

Review 8.  Tumour heterogeneity and metastasis at single-cell resolution.

Authors:  Devon A Lawson; Kai Kessenbrock; Ryan T Davis; Nicholas Pervolarakis; Zena Werb
Journal:  Nat Cell Biol       Date:  2018-11-26       Impact factor: 28.824

9.  Hedgehog-Gli activators direct osteo-chondrogenic function of bone morphogenetic protein toward osteogenesis in the perichondrium.

Authors:  Hironori Hojo; Shinsuke Ohba; Kiyomi Taniguchi; Masataka Shirai; Fumiko Yano; Taku Saito; Toshiyuki Ikeda; Keiji Nakajima; Yuske Komiyama; Naomi Nakagata; Kentaro Suzuki; Yuji Mishina; Masahisa Yamada; Tomohiro Konno; Tsuyoshi Takato; Hiroshi Kawaguchi; Hideki Kambara; Ung-il Chung
Journal:  J Biol Chem       Date:  2013-02-19       Impact factor: 5.157

10.  Identifying single-cell molecular programs by stochastic profiling.

Authors:  Kevin A Janes; Chun-Chao Wang; Karin J Holmberg; Kristin Cabral; Joan S Brugge
Journal:  Nat Methods       Date:  2010-03-14       Impact factor: 28.547
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