| Literature DB >> 19520101 |
Jozi G Figueiredo1, Flávio S Bitencourt, Mário R L Mota, Priscila P Silvestre, Cibele N Aguiar, Raquel G Benevides, Kyria S Nascimento, Tales R de Moura, Daniela Dal-Secco, Ana M S Assreuy, Fernando de Q Cunha, Marcus R Vale, Benildo S Cavada, Nylane M N Alencar.
Abstract
In the present study, we investigated the involvement of resident cell and inflammatory mediators in the neutrophil migration induced by chemotactic activity of a glucose/mannose-specific lectin isolated from Dioclea rostrata seeds (DrosL). Rats were injected i.p. with DrosL (125-1000 microg/cavity), and at 2-96 h thereafter the leukocyte counts in peritoneal fluid were determined. DrosL-induced a dose-dependent neutrophil migration accumulation, which reached maximal response at 24 h after injection and declines thereafter. The carbohydrate ligand nearly abolished the neutrophil influx. Pre-treatment of peritoneal cavities with thioglycolate which increases peritoneal macrophage numbers, enhanced neutrophil migration induced by DrosL by 303%. However, the reduction of peritoneal mast cell numbers by treatment of the cavities with compound 48/80 did not modify DrosL-induced neutrophil migration. The injection into peritoneal cavities of supernatants from macrophage cultures stimulated with DrosL (125, 250 and 500 microg/ml) induced neutrophil migration. In addition, DrosL treatment induced cytokines (TNF-alpha, IL-1beta and CINC-1) and NO release into the peritoneal cavity of rats. Finally, neutrophil chemotaxis assay in vitro showed that the lectin (15 and 31 microg/ml) induced neutrophil chemotaxis by even 180%. In conclusion, neutrophil migration induced by D. rostrata lectin occurs by way of the release of NO and cytokines such as IL-1beta, TNF-alpha and CINC-1.Entities:
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Year: 2009 PMID： 19520101 DOI： 10.1016/j.toxicon.2009.05.037
Source DB: PubMed Journal: Toxicon ISSN： 0041-0101 Impact factor: 3.033