Literature DB >> 19495740

An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy.

Carel van Oven1, Przemek M Krawczyk, Jan Stap, Arline M Melo, Maria H O Piazzetta, Angelo L Gobbi, Henk A van Veen, Jan Verhoeven, Jacob A Aten.   

Abstract

Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.

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Year:  2009        PMID： 19495740      PMCID： PMC2701496          DOI： 10.1007/s00249-009-0472-7

Source DB:  PubMed          Journal:  Eur Biophys J        ISSN： 0175-7571            Impact factor:   1.733


  55 in total

1.  Local UV-induced DNA damage in cell nuclei results in local transcription inhibition.

Authors:  M J Moné; M Volker; O Nikaido; L H Mullenders; A A van Zeeland; P J Verschure; E M Manders; R van Driel
Journal:  EMBO Rep       Date:  2001-11       Impact factor: 8.807

2.  A new system for laser-UVA-microirradiation of living cells.

Authors:  J Walter; T Cremer; K Miyagawa; S Tashiro
Journal:  J Microsc       Date:  2003-02       Impact factor: 1.758

3.  Upgrading of the Gray Laboratory soft X ray microprobe and V79 survival measurements following irradiation of one or all cells with a CK X ray beam of different size.

Authors:  G Schettino; M Folkard; K M Prise; B Vojnovic; B D Michael
Journal:  Radiat Prot Dosimetry       Date:  2002       Impact factor: 0.972

4.  Analysis of mouse Rad54 expression and its implications for homologous recombination.

Authors:  Jeroen Essers; Rudi W Hendriks; Joanna Wesoly; Cecile E M T Beerens; Bep Smit; Jan H J Hoeijmakers; Claire Wyman; Mies L G Dronkert; Roland Kanaar
Journal:  DNA Repair (Amst)       Date:  2002-10-01

5.  Distribution and repair of bipyrimidine photoproducts in solar UV-irradiated mammalian cells. Possible role of Dewar photoproducts in solar mutagenesis.

Authors:  D Perdiz; P Grof; M Mezzina; O Nikaido; E Moustacchi; E Sage
Journal:  J Biol Chem       Date:  2000-09-01       Impact factor: 5.157

6.  A comparison of cytotoxicity after whole- or partial-cell irradiation with synchrotron-produced ultrasoft X rays.

Authors:  B E Nelms; T R Mackie; J F MacKay; C K Hill; P M DeLuca; M J Lindstrom; J Deasy; M N Gould
Journal:  Radiat Res       Date:  1998-11       Impact factor: 2.841

7.  DNA replication but not nucleotide excision repair is required for UVC-induced replication protein A phosphorylation in mammalian cells.

Authors:  G Rodrigo; S Roumagnac; M S Wold; B Salles; P Calsou
Journal:  Mol Cell Biol       Date:  2000-04       Impact factor: 4.272

8.  MDC1 is a mediator of the mammalian DNA damage checkpoint.

Authors:  Grant S Stewart; Bin Wang; Colin R Bignell; A Malcolm R Taylor; Stephen J Elledge
Journal:  Nature       Date:  2003-02-27       Impact factor: 49.962

9.  Comments on the recently reported low biological effectiveness of ultrasoft X rays.

Authors:  M A Hill; D L Stevens; K M Stuart Townsend; D T Goodhead
Journal:  Radiat Res       Date:  2001-03       Impact factor: 2.841

10.  Dependence of the yield of DNA double-strand breaks in Chinese hamster V79-4 cells on the photon energy of ultrasoft X rays.

Authors:  C M de Lara; M A Hill; T J Jenner; D Papworth; P O'Neill
Journal:  Radiat Res       Date:  2001-03       Impact factor: 2.841
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  7 in total

Review 1.  Microirradiation techniques in radiobiological research.

Authors:  Guido A Drexler; Miguel J Ruiz-Gómez
Journal:  J Biosci       Date:  2015-09       Impact factor: 1.826

2.  Creating localized DNA double-strand breaks with microirradiation.

Authors:  Keiji Suzuki; Motohiro Yamauchi; Yasuyoshi Oka; Masatoshi Suzuki; Shunichi Yamashita
Journal:  Nat Protoc       Date:  2011-01-13       Impact factor: 13.491

3.  Characterization and implementation of a miniature X-ray system for live cell microscopy.

Authors:  Surendra Prajapati; Maëlle Locatelli; Caleb Sawyer; Julia Holmes; Keith Bonin; Paul Black; Pierre-Alexandre Vidi
Journal:  Mutat Res       Date:  2021-12-09       Impact factor: 3.151

4.  A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.

Authors:  Keiji Suzuki; Motohiro Yamauchi; Yasuyoshi Oka; Masatoshi Suzuki; Shunichi Yamashita
Journal:  Nucleic Acids Res       Date:  2010-04-12       Impact factor: 16.971

5.  Meta-analysis of DNA double-strand break response kinetics.

Authors:  Jakub A Kochan; Emilie C B Desclos; Ruben Bosch; Luna Meister; Lianne E M Vriend; Haico van Attikum; Przemek M Krawczyk
Journal:  Nucleic Acids Res       Date:  2017-12-15       Impact factor: 16.971

6.  Ultra-soft X-ray system for imaging the early cellular responses to X-ray induced DNA damage.

Authors:  Jakub A Kochan; Matthias van den Belt; Julia von der Lippe; Emilie C B Desclos; Barbara Steurer; Ron A Hoebe; Enzo M Scutigliani; Jan Verhoeven; Jan Stap; Ruben Bosch; Meindert Rijpkema; Carel van Oven; Henk A van Veen; Irene Stellingwerf; Lianne E M Vriend; Jurgen A Marteijn; Jacob A Aten; Przemek M Krawczyk
Journal:  Nucleic Acids Res       Date:  2019-09-26       Impact factor: 16.971

7.  Recruitment kinetics of DNA repair proteins Mdc1 and Rad52 but not 53BP1 depend on damage complexity.

Authors:  Volker Hable; Guido A Drexler; Tino Brüning; Christian Burgdorf; Christoph Greubel; Anja Derer; Judith Seel; Hilmar Strickfaden; Thomas Cremer; Anna A Friedl; Günther Dollinger
Journal:  PLoS One       Date:  2012-07-30       Impact factor: 3.240
  7 in total

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