Respiratory phase-correlated micro-CT imaging of free-breathing rodents.

We provide a dedicated phase-correlated imaging procedure for respiratory gating in micro-CT imaging with automatic detection of the optimal data window providing the least amount of motion blurring. A rawdata-based motion function (kymogram) was used for synchronization purposes and for identification of the optimal data window used for phase-correlated image reconstruction. Measurements were performed on a dual-source micro-CT scanner. Projection data were acquired over ten rotations for multi-segment phase-correlated reconstruction. Visual assessment was performed on datasets of ten free-breathing subjects. The kymogram approach provided a reliable synchronization signal for phase-correlated image reconstruction. Also, it allowed for the identification of phase intervals of increased and decreased motion and the corresponding detection of the optimal reconstruction phase. Phase-correlated images showed a strong improvement with respect to motion blurring compared to standard image reconstruction. A reconstruction for the calculated optimal data window provided the least amount of motion blurring and even allowed for the assessment of small structures in the lung. The dedicated retrospective phase-correlated image reconstruction procedure for respiratory gating is a feasible approach for motion-free imaging. A subject-specific optimal reconstruction phase can minimize motion blurring and further improve image quality.