Literature DB >> 19488869

Comparative genomic hybridization on spotted oligonucleotide microarrays.

Young H Kim1, Jonathan R Pollack.   

Abstract

Recent advances in DNA microarray technology have enabled researchers to comprehensively characterize the complex genomes of higher eukaryotic organisms at an unprecedented level of detail. Array-based comparative genomic hybridization (Array-CGH) has been widely used for detecting DNA copy number alterations on a genomic scale, where the mapping resolution is limited only by the number of probes on the DNA microarray. In this chapter, we present a validated protocol utilizing print-tip spotted HEEBO (Human Exonic Evidence Based Oligonucleotide) microarrays for conducting array-CGH using as little as 25 ng of genomic DNA from a wide variety of sources, including cultured cell lines and clinical specimens, with high spatial resolution and array-to-array reproducibility.

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Year:  2009        PMID： 19488869      PMCID： PMC4209297          DOI： 10.1007/978-1-60327-192-9_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

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3.  High resolution microarray comparative genomic hybridisation analysis using spotted oligonucleotides.

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4.  Comparative genomic hybridization using oligonucleotide microarrays and total genomic DNA.

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Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-10       Impact factor: 11.205

5.  Quantitative monitoring of gene expression patterns with a complementary DNA microarray.

Authors:  M Schena; D Shalon; R W Davis; P O Brown
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Authors:  A A Hill; C P Hunter; B T Tsung; G Tucker-Kellogg; E L Brown
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7.  Genome-wide expression monitoring in Saccharomyces cerevisiae.

Authors:  L Wodicka; H Dong; M Mittmann; M H Ho; D J Lockhart
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Journal:  Science       Date:  1999-10-15       Impact factor: 47.728

9.  Representational oligonucleotide microarray analysis: a high-resolution method to detect genome copy number variation.

Authors:  Robert Lucito; John Healy; Joan Alexander; Andrew Reiner; Diane Esposito; Maoyen Chi; Linda Rodgers; Amy Brady; Jonathan Sebat; Jennifer Troge; Joseph A West; Seth Rostan; Ken C Q Nguyen; Scott Powers; Kenneth Q Ye; Adam Olshen; Ennapadam Venkatraman; Larry Norton; Michael Wigler
Journal:  Genome Res       Date:  2003-09-15       Impact factor: 9.043

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Journal:  Oncogene       Date:  2008-01-21       Impact factor: 9.867
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