AIM: To develop novel DNA extraction and typing procedure for DNA identification of the 7th century human remains, determine the familiar relationship between the individuals, estimate the Y-chromosome haplogroup, and compare the Y-chromosome haplotype with the contemporary populations. METHODS: DNA from preserved femur samples was extracted using the modified silica-based extraction technique. Polymerase chain reaction amplification was performed using human identification kits MiniFiler, Identifiler, and Y-filer and also laboratory-developed and validated Y-chromosome short tandem repeat (STR) pentaplexes with short amplicons. RESULTS: For 244A, 244B, 244C samples, full autosomal DNA profiles (15 STR markers and Amelogenin) and for 244D, 244E, 244F samples, MiniFiler profiles were produced. Y-chromosome haplotypes consisting of up to 24 STR markers were determined and used to predict the Y-chromosome haplogroups and compare the resulting haplotypes with the current population. Samples 244A, 244B, 244C, and 244D belong to Y-chromosome haplogroup R1b and the samples 244E and 244F to haplogroup G2a. Comparison of ancient haplotypes with the current population yielded numerous close matches with genetic distance below 2. CONCLUSION: Application of forensic genetics in archaeology enables retrieving new types of information and helps in data interpretation. The number of successfully typed autosomal and Y-STR loci from ancient specimens in this study is one of the largest published so far for aged samples.
AIM: To develop novel DNA extraction and typing procedure for DNA identification of the 7th century human remains, determine the familiar relationship between the individuals, estimate the Y-chromosome haplogroup, and compare the Y-chromosome haplotype with the contemporary populations. METHODS: DNA from preserved femur samples was extracted using the modified silica-based extraction technique. Polymerase chain reaction amplification was performed using human identification kits MiniFiler, Identifiler, and Y-filer and also laboratory-developed and validated Y-chromosome short tandem repeat (STR) pentaplexes with short amplicons. RESULTS: For 244A, 244B, 244C samples, full autosomal DNA profiles (15 STR markers and Amelogenin) and for 244D, 244E, 244F samples, MiniFiler profiles were produced. Y-chromosome haplotypes consisting of up to 24 STR markers were determined and used to predict the Y-chromosome haplogroups and compare the resulting haplotypes with the current population. Samples 244A, 244B, 244C, and 244D belong to Y-chromosome haplogroup R1b and the samples 244E and 244F to haplogroup G2a. Comparison of ancient haplotypes with the current population yielded numerous close matches with genetic distance below 2. CONCLUSION: Application of forensic genetics in archaeology enables retrieving new types of information and helps in data interpretation. The number of successfully typed autosomal and Y-STR loci from ancient specimens in this study is one of the largest published so far for aged samples.
Authors: P Taberlet; S Griffin; B Goossens; S Questiau; V Manceau; N Escaravage; L P Waits; J Bouvet Journal: Nucleic Acids Res Date: 1996-08-15 Impact factor: 16.971
Authors: Jaume García-Bour; Alejandro Pérez-Pérez; Sara Alvarez; Eva Fernández; Ana María López-Parra; Eduardo Arroyo-Pardo; Daniel Turbón Journal: Am J Phys Anthropol Date: 2004-04 Impact factor: 2.868
Authors: Patricia Balaresque; Emma J Parkin; Lutz Roewer; Denise R Carvalho-Silva; R John Mitchell; Roland A H van Oorschot; Jürgen Henke; Mark Stoneking; Ivan Nasidze; Jon Wetton; Peter de Knijff; Chris Tyler-Smith; Mark A Jobling Journal: Int J Legal Med Date: 2008-06-14 Impact factor: 2.686
Authors: Marie Lacan; Christine Keyser; François-Xavier Ricaut; Nicolas Brucato; Francis Duranthon; Jean Guilaine; Eric Crubézy; Bertrand Ludes Journal: Proc Natl Acad Sci U S A Date: 2011-05-31 Impact factor: 11.205
Authors: Daniel Vanek; Bruce Budowle; Jitka Dubska-Votrubova; Angie Ambers; Jan Frolik; Martin Pospisek; Ahmed Anwar Al Afeefi; Khalid Ismaeil Al Hosani; Marie Allen; Khudooma Saeed Al Naimi; Dina Al Salafi; Wafa Ali Rashid Al Tayyari; Wendy Arguetaa; Michel Bottinelli; Magdalena M Bus; Jan Cemper-Kiesslich; Olivier Cepil; Greet De Cock; Stijn Desmyter; Hamid El Amri; Hicham El Ossmani; Ruth Galdies; Sebastian Grün; Francois Guidet; Anna Hoefges; Cristian Bogdan Iancu; Petra Lotz; Alessandro Maresca; Marion Nagy; Jindrich Novotny; Hajar Rachid; Jessica Rothe; Marguerethe Stenersen; Mishel Stephenson; Alain Stevanovitch; Juliane Strien; Denilce R Sumita; Joanna Vella; Judith Zander Journal: Croat Med J Date: 2017-06-14 Impact factor: 1.351