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Literature DB >> 19465918

A toolkit for high-throughput, cross-species gene engineering in Drosophila.

Radoslaw K Ejsmont¹, Mihail Sarov, Sylke Winkler, Kamil A Lipinski, Pavel Tomancak.

Abstract

We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.

Entities: Gene Species

Mesh：

Year: 2009 PMID： 19465918 DOI： 10.1038/nmeth.1334

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

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3. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.

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4. P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster.

Authors: Koen J T Venken; Yuchun He; Roger A Hoskins; Hugo J Bellen
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5. A new logic for DNA engineering using recombination in Escherichia coli.

Authors: Y Zhang; F Buchholz; J P Muyrers; A F Stewart
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6. An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases.

Authors: Johannes Bischof; Robert K Maeda; Monika Hediger; François Karch; Konrad Basler
Journal: Proc Natl Acad Sci U S A Date: 2007-02-22 Impact factor: 11.205

7. Recombineering-mediated tagging of Drosophila genomic constructs for in vivo localization and acute protein inactivation.

Authors: Koen J T Venken; Jaroslaw Kasprowicz; Sabine Kuenen; Jiekun Yan; Bassem A Hassan; Patrik Verstreken
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8. Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes.

Authors: Michele Markstein; Chrysoula Pitsouli; Christians Villalta; Susan E Celniker; Norbert Perrimon
Journal: Nat Genet Date: 2008-03-02 Impact factor: 38.330

9. Global analysis of patterns of gene expression during Drosophila embryogenesis.

Authors: Pavel Tomancak; Benjamin P Berman; Amy Beaton; Richard Weiszmann; Elaine Kwan; Volker Hartenstein; Susan E Celniker; Gerald M Rubin
Journal: Genome Biol Date: 2007 Impact factor: 13.583

10. Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.

Authors: Alexander W Bird; Anthony A Hyman
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64 in total

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Journal: Genetics Date: 2011-12-14 Impact factor: 4.562

2. Shaping cells and organs in Drosophila by opposing roles of fat body-secreted Collagen IV and perlecan.

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Journal: Dev Cell Date: 2011-08-16 Impact factor: 12.270

3. A molecularly defined duplication set for the X chromosome of Drosophila melanogaster.

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Review 4. Resources for functional genomics studies in Drosophila melanogaster.

Authors: Stephanie E Mohr; Yanhui Hu; Kevin Kim; Benjamin E Housden; Norbert Perrimon
Journal: Genetics Date: 2014-03-20 Impact factor: 4.562

5. Molecular biology: A fly in the face of genomics.

Authors: Eileen E M Furlong
Journal: Nature Date: 2011-03-24 Impact factor: 49.962

6. The Long 3'UTR mRNA of CaMKII Is Essential for Translation-Dependent Plasticity of Spontaneous Release in Drosophila melanogaster.

Authors: Elena A Kuklin; Stephen Alkins; Baskar Bakthavachalu; Maria C Genco; Indulekha Sudhakaran; K Vijay Raghavan; Mani Ramaswami; Leslie C Griffith
Journal: J Neurosci Date: 2017-09-27 Impact factor: 6.167