Literature DB >> 24576618

RNAi screening in Drosophila cells and in vivo.

Stephanie E Mohr1.   

Abstract

Here, I discuss how RNAi screening can be used effectively to uncover gene function. Specifically, I discuss the types of high-throughput assays that can be done in Drosophila cells and in vivo, RNAi reagent design and available reagent collections, automated screen pipelines, analysis of screen results, and approaches to RNAi results verification.
Copyright © 2014. Published by Elsevier Inc.

Entities:  

Keywords:  Cell-based assays; Drosophila; Functional genomics; High-throughput screening; In vivo assays; RNAi

Mesh：

Year:  2014        PMID： 24576618      PMCID： PMC4206080          DOI： 10.1016/j.ymeth.2014.02.018

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


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