Literature DB >> 19448747

Quantitative determination of urea concentrations in cell culture medium.

Robert J X Zawada1, Peggy Kwan, Kellen L Olszewski, Manuel Llinas, Shu-Gui Huang.   

Abstract

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.

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Year:  2009        PMID： 19448747      PMCID： PMC2804236          DOI： 10.1139/o09-011

Source DB:  PubMed          Journal:  Biochem Cell Biol        ISSN： 0829-8211            Impact factor:   3.626


  9 in total

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Journal:  Mol Cell Biochem       Date:  2003-02       Impact factor: 3.396

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  9 in total
  11 in total

1.  Self-Registration Methods for Increasing Membrane Utilization within Compression-Sealed Microchannel Hemodialysers.

Authors:  Brian K Paul; Spencer D Porter
Journal:  J Manuf Process       Date:  2014-10       Impact factor: 5.010

2.  Arginase overexpression in neurons and its effect on traumatic brain injury.

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Journal:  Mol Genet Metab       Date:  2018-07-25       Impact factor: 4.797

3.  Nitric-oxide supplementation for treatment of long-term complications in argininosuccinic aciduria.

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Journal:  Am J Hum Genet       Date:  2012-04-26       Impact factor: 11.025

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Authors:  Sashi Kant; Gangarao Davuluri; Khaled A Alchirazi; Nicole Welch; Claire Heit; Avinash Kumar; Mahesha Gangadhariah; Adam Kim; Megan R McMullen; Belinda Willard; Donal S Luse; Laura E Nagy; Vasilis Vasiliou; Anna Maria Marini; I David Weiner; Srinivasan Dasarathy
Journal:  J Biol Chem       Date:  2019-03-14       Impact factor: 5.157

5.  Synthesis, evaluation and molecular modelling of piceatannol analogues as arginase inhibitors.

Authors:  J Muller; B Cardey; A Zedet; C Desingle; M Grzybowski; P Pomper; S Foley; D Harakat; C Ramseyer; C Girard; M Pudlo
Journal:  RSC Med Chem       Date:  2020-04-17

6.  Generation of functional hepatocyte 3D discoids in an acoustofluidic bioreactor.

Authors:  Mogibelrahman M S Khedr; Walid Messaoudi; Umesh S Jonnalagadda; Ahmed M Abdelmotelb; Peter Glynne-Jones; Martyn Hill; Salim I Khakoo; Mohammed Abu Hilal
Journal:  Biomicrofluidics       Date:  2019-02-12       Impact factor: 2.800

7.  Enhanced productivity of a lutein-enriched novel acidophile microalga grown on urea.

Authors:  Carlos Casal; Maria Cuaresma; Jose Maria Vega; Carlos Vilchez
Journal:  Mar Drugs       Date:  2010-12-24       Impact factor: 5.118

8.  On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids.

Authors:  Giorgio Mattei; Chiara Magliaro; Serena Giusti; Sarada Devi Ramachandran; Stefan Heinz; Joris Braspenning; Arti Ahluwalia
Journal:  PLoS One       Date:  2017-03-07       Impact factor: 3.240

9.  Structural insights into human Arginase-1 pH dependence and its inhibition by the small molecule inhibitor CB-1158.

Authors:  Yvonne Grobben; Joost C M Uitdehaag; Nicole Willemsen-Seegers; Werner W A Tabak; Jos de Man; Rogier C Buijsman; Guido J R Zaman
Journal:  J Struct Biol X       Date:  2019-11-26

10.  Free Standing, Large-Area Silicon Nitride Membranes for High Toxin Clearance in Blood Surrogate for Small-Format Hemodialysis.

Authors:  Joshua J Miller; Jared A Carter; Kayli Hill; Jon-Paul S DesOrmeaux; Robert N Carter; Thomas R Gaborski; James A Roussie; James L McGrath; Dean G Johnson
Journal:  Membranes (Basel)       Date:  2020-06-06
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