| Literature DB >> 1137920 |
D Jung, H Biggs, J Erikson, P U Ledyard.
Abstract
A reaction of urea, o-phthalaldehyde and N-(1-naphthyl)ethylenediamine is described for measurement of urea by manual, continuous-flow, and kinetic methods. The continuous-flow system requires 25 mu-l of sample; 40 samples can be analyzed per hour. The kinetic method requires no enzymes, has no lag phase, and has good sensitivity. A major advantage of the reaction is that it occurs at a temperature of 37 degrees C or lower. The results obtained by all three methods agree well with those for a continuous-flow procedure in which diacetyl is a reagent.Entities:
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Year: 1975 PMID： 1137920
Source DB: PubMed Journal: Clin Chem ISSN： 0009-9147 Impact factor: 8.327