Literature DB >> 19427338

Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay.

Malena E Sandberg1, Denise Schellmann, Gerda Brunhofer, Thomas Erker, Igor Busygin, Reko Leino, Pia M Vuorela, Adyary Fallarero.   

Abstract

Staining of Staphylococcus aureus biofilms with 20 microM resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated.

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Year:  2009        PMID: 19427338     DOI: 10.1016/j.mimet.2009.04.014

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  27 in total

1.  In vitro activities of amoxicillin-clavulanate, doxycycline, ceftazidime, imipenem, and trimethoprim-sulfamethoxazole against biofilm of Brazilian strains of Burkholderia pseudomallei.

Authors:  Tereza de Jesus Pinheiro Gomes Bandeira; Camila Alencar Moreira; Raimunda Sâmia Nogueira Brilhante; Débora de Souza Collares Maia Castelo-Branco; Manoel Paiva de Araújo Neto; Rossana de Aguiar Cordeiro; Terezinha de Jesus Santos Rodrigues; Marcos Fábio Gadelha Rocha; José Júlio Costa Sidrim
Journal:  Antimicrob Agents Chemother       Date:  2013-09-03       Impact factor: 5.191

2.  A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries.

Authors:  Malena E Skogman; Pia M Vuorela; Adyary Fallarero
Journal:  J Vis Exp       Date:  2016-12-27       Impact factor: 1.355

3.  Activity of Antibiotics against Staphylococcus aureus in an In Vitro Model of Biofilms in the Context of Cystic Fibrosis: Influence of the Culture Medium.

Authors:  Yvan Diaz Iglesias; Tobias Wilms; Rita Vanbever; Françoise Van Bambeke
Journal:  Antimicrob Agents Chemother       Date:  2019-06-24       Impact factor: 5.191

4.  Effective Antibiofilm Polyketides against Staphylococcus aureus from the Pyranonaphthoquinone Biosynthetic Pathways of Streptomyces Species.

Authors:  Terhi Oja; Paola San Martin Galindo; Takaaki Taguchi; Suvi Manner; Pia M Vuorela; Koji Ichinose; Mikko Metsä-Ketelä; Adyary Fallarero
Journal:  Antimicrob Agents Chemother       Date:  2015-07-20       Impact factor: 5.191

5.  Phytochemical Analysis, Antioxidant, Antimicrobial, and Cytotoxic Activity of Different Extracts of Xanthoparmelia stenophylla Lichen from Stara Planina, Serbia.

Authors:  Aleksandar Kocovic; Jovana Jeremic; Jovana Bradic; Miroslav Sovrlic; Jovica Tomovic; Perica Vasiljevic; Marijana Andjic; Nevena Draginic; Mirjana Grujovic; Katarina Mladenovic; Dejan Baskic; Suzana Popovic; Sanja Matic; Vladimir Zivkovic; Nevena Jeremic; Vladimir Jakovljevic; Nedeljko Manojlovic
Journal:  Plants (Basel)       Date:  2022-06-21

6.  A combined pharmacodynamic quantitative and qualitative model reveals the potent activity of daptomycin and delafloxacin against Staphylococcus aureus biofilms.

Authors:  Julia Bauer; Wafi Siala; Paul M Tulkens; Françoise Van Bambeke
Journal:  Antimicrob Agents Chemother       Date:  2013-04-09       Impact factor: 5.191

7.  Systematic exploration of natural and synthetic flavonoids for the inhibition of Staphylococcus aureus biofilms.

Authors:  Suvi Manner; Malena Skogman; Darla Goeres; Pia Vuorela; Adyary Fallarero
Journal:  Int J Mol Sci       Date:  2013-09-25       Impact factor: 5.923

8.  Bacteria viability assessment after photocatalytic treatment.

Authors:  Yanling Cai; Maria Strømme; Ken Welch
Journal:  3 Biotech       Date:  2013-05-21       Impact factor: 2.406

9.  (+)-Dehydroabietic acid, an abietane-type diterpene, inhibits Staphylococcus aureus biofilms in vitro.

Authors:  Adyary Fallarero; Malena Skogman; Janni Kujala; Mohanathas Rajaratnam; Vânia M Moreira; Jari Yli-Kauhaluoma; Pia Vuorela
Journal:  Int J Mol Sci       Date:  2013-06-05       Impact factor: 5.923

10.  Liposomes-in-hydrogel delivery system with mupirocin: in vitro antibiofilm studies and in vivo evaluation in mice burn model.

Authors:  Julia Hurler; Karen K Sørensen; Adyary Fallarero; Pia Vuorela; Nataša Škalko-Basnet
Journal:  Biomed Res Int       Date:  2013-11-28       Impact factor: 3.411

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