Literature DB >> 19408299

Nonadditive effects of mixed crowding on protein stability.

Jyotica Batra1, Ke Xu, Huan-Xiang Zhou.   

Abstract

The crowded environments inside cells can have significant effects on the folding stability and other biophysical properties of proteins. In this study on how macromolecular crowding affects protein folding, we took a significant step toward realistically mimicking intracellular environments by using a mixture of two crowding agents, Ficoll and dextran. We found that the mixed crowding exerts a greater stabilizing effect than the sum of the two individual crowding agents. Therefore, the composition of crowders, not just the total concentration, has a significant influence on the effects of crowding on protein folding. Since the composition of intracellular macromolecules varies within the lifetime of a cell, our finding may provide an explanation for age being an important risk factor for protein aggregation-related diseases such as Alzheimer's disease and Parkinson's disease.

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Year:  2009        PMID： 19408299      PMCID： PMC4456354          DOI： 10.1002/prot.22425

Source DB:  PubMed          Journal:  Proteins        ISSN： 0887-3585


  22 in total

1.  Crowding effects on EcoRV kinetics and binding.

Authors:  J R Wenner; V A Bloomfield
Journal:  Biophys J       Date:  1999-12       Impact factor: 4.033

2.  Effect of a concentrated "inert" macromolecular cosolute on the stability of a globular protein with respect to denaturation by heat and by chaotropes: a statistical-thermodynamic model.

Authors:  A P Minton
Journal:  Biophys J       Date:  2000-01       Impact factor: 4.033

3.  Protein folding and binding in confined spaces and in crowded solutions.

Authors:  Huan-Xiang Zhou
Journal:  J Mol Recognit       Date:  2004 Sep-Oct       Impact factor: 2.137

4.  Molecular crowding enhances native state stability and refolding rates of globular proteins.

Authors:  Margaret S Cheung; Dmitri Klimov; D Thirumalai
Journal:  Proc Natl Acad Sci U S A       Date:  2005-03-21       Impact factor: 11.205

5.  Models for excluded volume interaction between an unfolded protein and rigid macromolecular cosolutes: macromolecular crowding and protein stability revisited.

Authors:  Allen P Minton
Journal:  Biophys J       Date:  2004-12-13       Impact factor: 4.033

6.  Effect of mixed macromolecular crowding agents on protein folding.

Authors:  Huan-Xiang Zhou
Journal:  Proteins       Date:  2008-09

Review 7.  Macromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences.

Authors:  Huan-Xiang Zhou; Germán Rivas; Allen P Minton
Journal:  Annu Rev Biophys       Date:  2008       Impact factor: 12.981

8.  Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: implications for protein folding in physiological environments.

Authors:  Fen Du; Zheng Zhou; Zhong-Ying Mo; Jun-Zhi Shi; Jie Chen; Yi Liang
Journal:  J Mol Biol       Date:  2006-09-12       Impact factor: 5.469

9.  15N NMR spin relaxation dispersion study of the molecular crowding effects on protein folding under native conditions.

Authors:  Xuanjun Ai; Zheng Zhou; Yawen Bai; Wing-Yiu Choy
Journal:  J Am Chem Soc       Date:  2006-03-29       Impact factor: 15.419

10.  Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli.

Authors:  S B Zimmerman; S O Trach
Journal:  J Mol Biol       Date:  1991-12-05       Impact factor: 5.469
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  37 in total

1.  The folding transition-state ensemble of a four-helix bundle protein: helix propensity as a determinant and macromolecular crowding as a probe.

Authors:  Harianto Tjong; Huan-Xiang Zhou
Journal:  Biophys J       Date:  2010-05-19       Impact factor: 4.033

2.  Generalized fundamental measure theory for atomistic modeling of macromolecular crowding.

Authors:  Sanbo Qin; Huan-Xiang Zhou
Journal:  Phys Rev E Stat Nonlin Soft Matter Phys       Date:  2010-03-26

3.  Brownian dynamics simulation of protein solutions: structural and dynamical properties.

Authors:  Paolo Mereghetti; Razif R Gabdoulline; Rebecca C Wade
Journal:  Biophys J       Date:  2010-12-01       Impact factor: 4.033

4.  Quantification of excluded volume effects on the folding landscape of Pseudomonas aeruginosa apoazurin in vitro.

Authors:  Alexander Christiansen; Pernilla Wittung-Stafshede
Journal:  Biophys J       Date:  2013-10-01       Impact factor: 4.033

5.  Effect of macromolecular crowding on protein binding stability: modest stabilization and significant biological consequences.

Authors:  Jyotica Batra; Ke Xu; Sanbo Qin; Huan-Xiang Zhou
Journal:  Biophys J       Date:  2009-08-05       Impact factor: 4.033

6.  Atomistic modeling of macromolecular crowding predicts modest increases in protein folding and binding stability.

Authors:  Sanbo Qin; Huan-Xiang Zhou
Journal:  Biophys J       Date:  2009-07-08       Impact factor: 4.033

7.  Power-law dependence of the melting temperature of ubiquitin on the volume fraction of macromolecular crowders.

Authors:  Matthias M Waegele; Feng Gai
Journal:  J Chem Phys       Date:  2011-03-07       Impact factor: 3.488

8.  Effects of macromolecular crowding on the inhibition of virus assembly and virus-cell receptor recognition.

Authors:  Verónica Rincón; Rebeca Bocanegra; Alicia Rodríguez-Huete; Germán Rivas; Mauricio G Mateu
Journal:  Biophys J       Date:  2011-02-02       Impact factor: 4.033

Review 9.  Protein-protein interactions in a crowded environment.

Authors:  Apratim Bhattacharya; Young C Kim; Jeetain Mittal
Journal:  Biophys Rev       Date:  2013-04-16

10.  A didactic model of macromolecular crowding effects on protein folding.

Authors:  Douglas Tsao; Allen P Minton; Nikolay V Dokholyan
Journal:  PLoS One       Date:  2010-08-03       Impact factor: 3.240
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