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Literature DB >> 19403760

Reverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis E virus.

Xi Lan¹, Bin Yang, Bao Yu Li, Xiang Ping Yin, Xue Rui Li, Ji Xing Liu.

Abstract

The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).

Entities: Chemical

Mesh：

Substances：
RNA, Viral

Year: 2009 PMID： 19403760 PMCID： PMC2708515 DOI： 10.1128/JCM.00498-09

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

13 in total

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Authors: X J Meng
Journal: J Hepatol Date: 2000-11 Impact factor: 25.083

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Authors: T Notomi; H Okayama; H Masubuchi; T Yonekawa; K Watanabe; N Amino; T Hase
Journal: Nucleic Acids Res Date: 2000-06-15 Impact factor: 16.971

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Authors: G G Schlauder; I K Mushahwar
Journal: J Med Virol Date: 2001-10 Impact factor: 2.327

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Journal: J Viral Hepat Date: 1997-01 Impact factor: 3.728

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Authors: R Aggarwal; K Krawczynski
Journal: J Gastroenterol Hepatol Date: 2000-01 Impact factor: 4.029

6. Complete or near-complete nucleotide sequences of hepatitis E virus genome recovered from a wild boar, a deer, and four patients who ate the deer.

Authors: Kazuaki Takahashi; Naoto Kitajima; Natsumi Abe; Shunji Mishiro
Journal: Virology Date: 2004-12-20 Impact factor: 3.616

7. Phylogenetic and case-control study on hepatitis E virus infection in Germany.

Authors: Ole Wichmann; Sven Schimanski; Judith Koch; Martin Kohler; Camilla Rothe; Annelie Plentz; Wolfgang Jilg; Klaus Stark
Journal: J Infect Dis Date: 2008-12-15 Impact factor: 5.226

8. Fulminant hepatic failure in pregnant women: acute fatty liver or acute viral hepatitis?

Authors: S S Hamid; S M Jafri; H Khan; H Shah; Z Abbas; H Fields
Journal: J Hepatol Date: 1996-07 Impact factor: 25.083

9. Presence of hepatitis E virus in sewage in Northern India: frequency and seasonal pattern.

Authors: Sirish Kumar Ippagunta; Sita Naik; Bechan Sharma; Rakesh Aggarwal
Journal: J Med Virol Date: 2007-12 Impact factor: 2.327

10. Outbreak of acute viral hepatitis due to hepatitis E virus in Hyderabad.

Authors: P Sarguna; A Rao; K N Sudha Ramana
Journal: Indian J Med Microbiol Date: 2007-10 Impact factor: 0.985

11 in total

Review 1. Diagnosis of hepatitis E.

Authors: Rakesh Aggarwal
Journal: Nat Rev Gastroenterol Hepatol Date: 2012-10-02 Impact factor: 46.802

Review 2. Hepatitis E virus infection.

Authors: Nassim Kamar; Harry R Dalton; Florence Abravanel; Jacques Izopet
Journal: Clin Microbiol Rev Date: 2014-01 Impact factor: 26.132

3. Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance.

Authors: Sally A Baylis; Kay-Martin Hanschmann; Johannes Blümel; C Micha Nübling
Journal: J Clin Microbiol Date: 2011-02-09 Impact factor: 5.948

Review 4. Autochthonous hepatitis e virus infections: a new transfusion-associated risk?

Authors: Jens Dreier; David Juhl
Journal: Transfus Med Hemother Date: 2013-12-30 Impact factor: 3.747

Review 5. Implications of Hepatitis E Virus in Blood Transfusions, Hemodialysis, and Solid Organ Transplants.

Authors: Essam M Janahi; Saba F D Parkar; Sakina Mustafa; Zaki M Eisa
Journal: Medicina (Kaunas) Date: 2020-04-25 Impact factor: 2.430

Review 6. A Review of the Diagnosis and Management of Hepatitis E.

Authors: P Kar; R Karna
Journal: Curr Treat Options Infect Dis Date: 2020-07-17

7. World Health Organization International Standard to harmonize assays for detection of hepatitis E virus RNA.

Authors: Sally A Baylis; Johannes Blümel; Saeko Mizusawa; Keiji Matsubayashi; Hidekatsu Sakata; Yoshiaki Okada; C Micha Nübling; Kay-Martin O Hanschmann
Journal: Emerg Infect Dis Date: 2013-05 Impact factor: 6.883

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Authors: Santiago Mirazo; Natalia Ramos; Victoria Mainardi; Solange Gerona; Juan Arbiza
Journal: Hepat Med Date: 2014-06-03

9. Rapid and real-time detection of Porcine Sapelovirus by reverse transcription loop-mediated isothermal amplification assay.

Authors: Chunyan Wang; Dayi Yu; Li Cui; Xiuguo Hua; Congli Yuan; Huan Sun; Yuxiao Liu
Journal: J Virol Methods Date: 2014-03-22 Impact factor: 2.014

10. Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses.

Authors: Ken-Ichi Hanaki; Fumio Ike; Rika Hatakeyama; Norio Hirano
Journal: J Virol Methods Date: 2012-10-30 Impact factor: 2.014