Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Silencing an inhibitor unleashes a cytotoxic enzyme.

Literature DB >> 19402708

Silencing an inhibitor unleashes a cytotoxic enzyme.

Abstract

The ribonuclease inhibitor (RI) is a cytosolic protein and a potent inhibitor of bovine pancreatic ribonuclease (RNase A). Amphibian homologues and variants of RNase A that evade RI are cytotoxic. Here, we employ RNA interference along with amphibian and mammalian ribonucleases to demonstrate that RI protects cells against exogenous ribonucleases. These data indicate an imperative for the molecular evolution of RI and suggest a means of enhancing the cytotoxicity of mammalian ribonucleases.

Entities: CellLine Chemical Disease Gene Mutation Species

Mesh：

Substances：

Year: 2009 PMID： 19402708 PMCID： PMC2802652 DOI： 10.1021/bi900489y

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

32 in total

Review 1. Protein-protein interfaces: mimics and inhibitors.

Authors: A G Cochran
Journal: Curr Opin Chem Biol Date: 2001-12 Impact factor: 8.822

Review 2. Macromolecular crowding: obvious but underappreciated.

Authors: R J Ellis
Journal: Trends Biochem Sci Date: 2001-10 Impact factor: 13.807

3. Compensating effects on the cytotoxicity of ribonuclease A variants.

Authors: Kimberly A Dickson; Caroline L Dahlberg; Ronald T Raines
Journal: Arch Biochem Biophys Date: 2003-07-15 Impact factor: 4.013

4. Creation of a zymogen.

Authors: Parit Plainkum; Stephen M Fuchs; Suthep Wiyakrutta; Ronald T Raines
Journal: Nat Struct Biol Date: 2003-02

5. A ribonuclease A variant with low catalytic activity but high cytotoxicity.

Authors: L E Bretscher; R L Abel; R T Raines
Journal: J Biol Chem Date: 2000-04-07 Impact factor: 5.157

6. Endowing human pancreatic ribonuclease with toxicity for cancer cells.

Authors: P A Leland; K E Staniszewski; B M Kim; R T Raines
Journal: J Biol Chem Date: 2001-09-12 Impact factor: 5.157

7. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems.

Authors: N J Caplen; S Parrish; F Imani; A Fire; R A Morgan
Journal: Proc Natl Acad Sci U S A Date: 2001-07-31 Impact factor: 11.205

8. Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells.

Authors: Peter C Scacheri; Orit Rozenblatt-Rosen; Natasha J Caplen; Tyra G Wolfsberg; Lowell Umayam; Jeffrey C Lee; Christina M Hughes; Kalai Selvi Shanmugam; Arindam Bhattacharjee; Matthew Meyerson; Francis S Collins
Journal: Proc Natl Acad Sci U S A Date: 2004-02-09 Impact factor: 11.205

Silencing an inhibitor unleashes a cytotoxic enzyme.

Review 1. Protein-protein interfaces: mimics and inhibitors.

Review 2. Macromolecular crowding: obvious but underappreciated.

3. Compensating effects on the cytotoxicity of ribonuclease A variants.

4. Creation of a zymogen.

5. A ribonuclease A variant with low catalytic activity but high cytotoxicity.

6. Endowing human pancreatic ribonuclease with toxicity for cancer cells.

7. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems.

8. Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells.

9. Interaction of onconase with the human ribonuclease inhibitor protein.

10. Ribonuclease inhibitor as an intracellular sentry.

1. Knockout of the Ribonuclease Inhibitor Gene Leaves Human Cells Vulnerable to Secretory Ribonucleases.

2. Cellular uptake of ribonuclease A relies on anionic glycans.

Review 3. Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.