Literature DB >> 19390151

Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde.

Matthew W Vetting1, Subray S Hegde, John S Blanchard.   

Abstract

The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and the strong reducing agent borane-dimethylamine resulted in a derivatized protein which produced crystals that diffracted to 1.6 A resolution; their structure was subsequently determined by single-wavelength anomalous dispersion. Analysis of the derivatized protein using Fourier transform ion cyclotron resonance mass spectrometry indicated a mass increase of 68 Da per free amino group. Electron-density maps about a limited number of structurally ordered lysines indicated that the modification was a cyclic pentylation of free amines, producing piperidine groups.

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Year:  2009        PMID： 19390151      PMCID： PMC2672816          DOI： 10.1107/S0907444909008324

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  34 in total

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Journal:  J Synchrotron Radiat       Date:  2003-11-28       Impact factor: 2.616

4.  The impact of Lys-->Arg surface mutations on the crystallization of the globular domain of RhoGDI.

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-01-23

5.  wARP: improvement and extension of crystallographic phases by weighted averaging of multiple-refined dummy atomic models.

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7.  The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins.

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Review 8.  X-ray diffraction studies on enzymes in the glycolytic pathway.

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Review 9.  The integration of macromolecular diffraction data.

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Review 10.  Scaling and assessment of data quality.

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  9 in total

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Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-11-25

2.  Structural and biochemical analysis of the pentapeptide repeat protein EfsQnr, a potent DNA gyrase inhibitor.

Authors:  Subray S Hegde; Matthew W Vetting; Lesley A Mitchenall; Anthony Maxwell; John S Blanchard
Journal:  Antimicrob Agents Chemother       Date:  2010-10-11       Impact factor: 5.191

3.  Pentapeptide-repeat proteins that act as topoisomerase poison resistance factors have a common dimer interface.

Authors:  Matthew W Vetting; Subray S Hegde; Yong Zhang; John S Blanchard
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-02-18

4.  Structural insights into quinolone antibiotic resistance mediated by pentapeptide repeat proteins: conserved surface loops direct the activity of a Qnr protein from a gram-negative bacterium.

Authors:  Xiaoli Xiong; Elizabeth H C Bromley; Peter Oelschlaeger; Derek N Woolfson; James Spencer
Journal:  Nucleic Acids Res       Date:  2011-01-11       Impact factor: 16.971

5.  Stabilization of porcine pancreatic elastase crystals by glutaraldehyde cross-linking.

Authors:  Stefan Hofbauer; José A Brito; Jalmira Mulchande; Przemyslaw Nogly; Miguel Pessanha; Rui Moreira; Margarida Archer
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Review 6.  Current Understanding of the Structure and Function of Pentapeptide Repeat Proteins.

Authors:  Ruojing Zhang; Michael A Kennedy
Journal:  Biomolecules       Date:  2021-04-26

7.  A mutational analysis and molecular dynamics simulation of quinolone resistance proteins QnrA1 and QnrC from Proteus mirabilis.

Authors:  Qinglan Guo; Jingwei Weng; Xiaogang Xu; Minghua Wang; Xiaoying Wang; Xinyu Ye; Wenning Wang; Minggui Wang
Journal:  BMC Struct Biol       Date:  2010-10-08

8.  Microbatch under-oil salt screening of organic cations: single-crystal growth of active pharmaceutical ingredients.

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9.  A novel secondary structure based on fused five-membered rings motif.

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Journal:  Sci Rep       Date:  2016-08-11       Impact factor: 4.379
  9 in total

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