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Literature DB >> 1938970

Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm.

Abstract

The cysteines of the Escherichia coli periplasmic enzyme alkaline phosphatase, which are involved in disulfide bonds in the native enzyme, were found to be fully reduced when the protein was retained in the cytoplasm. Under these circumstances the cysteines remained reduced for at least several minutes after the synthesis of the protein was completed. This contrasted with the normally exported protein, wherein disulfide bonds formed rapidly. Disulfide bond formation accompanied export and processing. The implications of these findings for the inactivity of the enzyme in the cytoplasm are discussed.

Entities: Chemical

Mesh：

Substances：

Year: 1991 PMID： 1938970 PMCID： PMC212545 DOI： 10.1128/jb.173.23.7719-7722.1991

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

22 in total

1. RNA editing in brain controls a determinant of ion flow in glutamate-gated channels.

Authors: B Sommer; M Köhler; R Sprengel; P H Seeburg
Journal: Cell Date: 1991-10-04 Impact factor: 41.582

2. Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.

Authors: C S Hoffman; A Wright
Journal: Proc Natl Acad Sci U S A Date: 1985-08 Impact factor: 11.205

3. Role of primary structure and disulfide bond formation in beta-lactamase secretion.

Authors: S Pollitt; H Zalkin
Journal: J Bacteriol Date: 1983-01 Impact factor: 3.490

4. Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins?

Authors: K Ito; P J Bassford; J Beckwith
Journal: Cell Date: 1981-06 Impact factor: 41.582

5. Disulphide bridges in globular proteins.

Authors: J M Thornton
Journal: J Mol Biol Date: 1981-09-15 Impact factor: 5.469

6. Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.

Authors: H Inouye; J Beckwith
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

7. The biosynthesis of rat serum albumin. In vivo studies on the formation of the disulfide bonds.

Authors: T Peters; L K Davidson
Journal: J Biol Chem Date: 1982-08-10 Impact factor: 5.157

8. 65Zn(II), 115mCd(II), 60Co(II), and mg(II) binding to alkaline phosphatase of Escherichia coli. Structural and functional effects.

Authors: J E Coleman; K Nakamura; J F Chlebowski
Journal: J Biol Chem Date: 1983-01-10 Impact factor: 5.157

9. Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

Authors: S Michaelis; H Inouye; D Oliver; J Beckwith
Journal: J Bacteriol Date: 1983-04 Impact factor: 3.490

10. TnphoA: a transposon probe for protein export signals.

Authors: C Manoil; J Beckwith
Journal: Proc Natl Acad Sci U S A Date: 1985-12 Impact factor: 11.205

76 in total

1. Mutational analysis and membrane topology of ComP, a quorum-sensing histidine kinase of Bacillus subtilis controlling competence development.

Authors: F Piazza; P Tortosa; D Dubnau
Journal: J Bacteriol Date: 1999-08 Impact factor: 3.490

2. Membrane topology of the NixA nickel transporter of Helicobacter pylori: two nickel transport-specific motifs within transmembrane helices II and III.

Authors: J F Fulkerson; H L Mobley
Journal: J Bacteriol Date: 2000-03 Impact factor: 3.490

Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm.

1. RNA editing in brain controls a determinant of ion flow in glutamate-gated channels.

2. Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.

3. Role of primary structure and disulfide bond formation in beta-lactamase secretion.

4. Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins?

5. Disulphide bridges in globular proteins.

6. Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.

7. The biosynthesis of rat serum albumin. In vivo studies on the formation of the disulfide bonds.

8. 65Zn(II), 115mCd(II), 60Co(II), and mg(II) binding to alkaline phosphatase of Escherichia coli. Structural and functional effects.

9. Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

10. TnphoA: a transposon probe for protein export signals.

1. Mutational analysis and membrane topology of ComP, a quorum-sensing histidine kinase of Bacillus subtilis controlling competence development.

2. Membrane topology of the NixA nickel transporter of Helicobacter pylori: two nickel transport-specific motifs within transmembrane helices II and III.

Review 3. Membrane topology and insertion of membrane proteins: search for topogenic signals.

4. Folding and activity of circularly permuted forms of a polytopic membrane protein.

5. Dislocation of membrane proteins in FtsH-mediated proteolysis.

6. Bacillus subtilis CcdA-defective mutants are blocked in a late step of cytochrome c biogenesis.

Review 7. The thioredoxin superfamily: redundancy, specificity, and gray-area genomics.

8. Disulfide bond formation and activation of Escherichia coli β-galactosidase under oxidizing conditions.

9. Membrane topology analysis of cyclic glucan synthase, a virulence determinant of Brucella abortus.

10. Probing the transmembrane topology of cyclic nucleotide-gated ion channels with a gene fusion approach.