| Literature DB >> 19378999 |
Zbigniew Pianowski1, Katarzyna Gorska, Laurence Oswald, Christoph A Merten, Nicolas Winssinger.
Abstract
Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells.Entities:
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Year: 2009 PMID： 19378999 DOI： 10.1021/ja809656k
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419