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Literature DB >> 19377038

Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae.

D Tielker¹, I Eichhof, K-E Jaeger, J F Ernst.

Abstract

Hypoxia is encountered frequently by pathogenic and apathogenic fungi. A codon-adapted gene encoding flavin mononucleotide-based fluorescent protein (CaFbFP) was expressed in Candida albicans and Saccharomyces cerevisiae. Both species produced CaFbFP and fluoresced even during hypoxia, suggesting that oxygen-independent CaFbFP is a useful, novel tool for monitoring hypoxic gene expression in fungi.

Entities: Chemical Disease Species

Mesh：

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Year: 2009 PMID： 19377038 PMCID： PMC2698303 DOI： 10.1128/EC.00394-08

Source DB: PubMed Journal: Eukaryot Cell ISSN： 1535-9786

12 in total

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Journal: Proc Natl Acad Sci U S A Date: 2004-08-06 Impact factor: 11.205

2. Reporter proteins for in vivo fluorescence without oxygen.

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3. In vivo and in vitro anaerobic mating in Candida albicans.

Authors: Raluca Dumitru; Dhammika H M L P Navarathna; Camile P Semighini; Christian G Elowsky; Razvan V Dumitru; Daniel Dignard; Malcolm Whiteway; Audrey L Atkin; Kenneth W Nickerson
Journal: Eukaryot Cell Date: 2007-01-26

Review 4. Advances in fluorescent protein technology.

Authors: Nathan C Shaner; George H Patterson; Michael W Davidson
Journal: J Cell Sci Date: 2007-12-15 Impact factor: 5.285

5. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression.

Authors: D Mumberg; R Müller; M Funk
Journal: Nucleic Acids Res Date: 1994-12-25 Impact factor: 16.971

6. Chlamydospore formation in Candida albicans requires the Efg1p morphogenetic regulator.

Authors: A Sonneborn; D P Bockmühl; J F Ernst
Journal: Infect Immun Date: 1999-10 Impact factor: 3.441

Review 7. Responses to hypoxia in fungal pathogens.

Authors: Joachim F Ernst; Denis Tielker
Journal: Cell Microbiol Date: 2008-11-03 Impact factor: 3.715

8. Hypoxia and extraintestinal dissemination of Candida albicans yeast forms.

Authors: Adam S Kim; Robb M Garni; Michelle J Henry-Stanley; Catherine M Bendel; Stanley L Erlandsen; Carol L Wells
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9. The CUG codon is decoded in vivo as serine and not leucine in Candida albicans.

Authors: M A Santos; M F Tuite
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10. Self-regulation of Candida albicans population size during GI colonization.

Authors: Sarah Jane White; Ari Rosenbach; Paul Lephart; Diem Nguyen; Alana Benjamin; Saul Tzipori; Malcolm Whiteway; Joan Mecsas; Carol A Kumamoto
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6. Characterization of flavin binding in oxygen-independent fluorescent reporters.

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