Literature DB >> 22544258

Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system.

Kathrin Emmi Scholz1, Daniel Okrob, Benita Kopka, Alexander Grünberger, Martina Pohl, Karl-Erich Jaeger, Ulrich Krauss.   

Abstract

Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system.

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Year:  2012        PMID： 22544258      PMCID： PMC3416370          DOI： 10.1128/AEM.00582-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  The enzymatic transformation of water-insoluble reactants in nonaqueous solvents. Conversion of cholesterol to cholest-4-ene-3-one by a Nocardia sp. Reprinted from Biotechnology and Bioengineering, Vol. XVII, Pages 815-826 (1975).

Authors:  B C Buckland; P Dunnill; M D Lilly
Journal:  Biotechnol Bioeng       Date:  2000-03-20       Impact factor: 4.530

Review 2.  Industrial potential of organic solvent tolerant bacteria.

Authors:  Yogita N Sardessai; Saroj Bhosle
Journal:  Biotechnol Prog       Date:  2004 May-Jun

3.  Asymmetric reduction of ketones with recombinant E. coli whole cells in neat substrates.

Authors:  Andre Jakoblinnert; Radoslav Mladenov; Albert Paul; Fabrizio Sibilla; Ulrich Schwaneberg; Marion B Ansorge-Schumacher; Pablo Domínguez de María
Journal:  Chem Commun (Camb)       Date:  2011-10-17       Impact factor: 6.222

Review 4.  Industrial biotechnology for the production of bio-based chemicals--a cradle-to-grave perspective.

Authors:  Rajni Hatti-Kaul; Ulrika Törnvall; Linda Gustafsson; Pål Börjesson
Journal:  Trends Biotechnol       Date:  2007-01-17       Impact factor: 19.536

5.  Reporter proteins for in vivo fluorescence without oxygen.

Authors:  Thomas Drepper; Thorsten Eggert; Franco Circolone; Achim Heck; Ulrich Krauss; Jan-Karl Guterl; Marion Wendorff; Aba Losi; Wolfgang Gärtner; Karl-Erich Jaeger
Journal:  Nat Biotechnol       Date:  2007-03-11       Impact factor: 54.908

6.  An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an alpha/beta-hydrolase fold.

Authors:  Jennifer Andexer; Jan von Langermann; Annett Mell; Marco Bocola; Udo Kragl; Thorsten Eggert; Martina Pohl
Journal:  Angew Chem Int Ed Engl       Date:  2007       Impact factor: 15.336

Review 7.  Whole cell biocatalysis in nonconventional media.

Authors:  P Nikolova; O P Ward
Journal:  J Ind Microbiol       Date:  1993-02

8.  Utilization of hydrophobic bacterium Rhodococcus opacus B-4 as whole-cell catalyst in anhydrous organic solvents.

Authors:  Shiho Yamashita; Masafumi Satoi; Yoshihiro Iwasa; Kohsuke Honda; Yuka Sameshima; Takeshi Omasa; Junichi Kato; Hisao Ohtake
Journal:  Appl Microbiol Biotechnol       Date:  2006-11-23       Impact factor: 4.813

9.  Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta.

Authors:  Holger Bühler; Franz Effenberger; Siegfried Förster; Jürgen Roos; Harald Wajant
Journal:  Chembiochem       Date:  2003-03-03       Impact factor: 3.164

10.  Enzymatic reduction of ketones in "micro-aqueous" media catalyzed by ADH-A from Rhodococcus ruber.

Authors:  Gonzalo de Gonzalo; Iván Lavandera; Kurt Faber; Wolfgang Kroutil
Journal:  Org Lett       Date:  2007-05-01       Impact factor: 6.005
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  5 in total

1.  Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism.

Authors:  Bryan J Jones; Zsófia Bata; Romas J Kazlauskas
Journal:  ACS Catal       Date:  2017-05-15       Impact factor: 13.084

Review 2.  Microbial engineering for aldehyde synthesis.

Authors:  Aditya M Kunjapur; Kristala L J Prather
Journal:  Appl Environ Microbiol       Date:  2015-01-09       Impact factor: 4.792

3.  Fusion of a flavin-based fluorescent protein to hydroxynitrile lyase from Arabidopsis thaliana improves enzyme stability.

Authors:  Kathrin Emmi Scholz; Benita Kopka; Astrid Wirtz; Martina Pohl; Karl-Erich Jaeger; Ulrich Krauss
Journal:  Appl Environ Microbiol       Date:  2013-05-31       Impact factor: 4.792

4.  Hydroxynitrile lyases from cyanogenic millipedes: molecular cloning, heterologous expression, and whole-cell biocatalysis for the production of (R)-mandelonitrile.

Authors:  Takuya Yamaguchi; Aem Nuylert; Atsutoshi Ina; Tsutomu Tanabe; Yasuhisa Asano
Journal:  Sci Rep       Date:  2018-02-14       Impact factor: 4.379

Review 5.  Applied biocatalysis beyond just buffers - from aqueous to unconventional media. Options and guidelines.

Authors:  Morten M C H van Schie; Jan-Dirk Spöring; Marco Bocola; Pablo Domínguez de María; Dörte Rother
Journal:  Green Chem       Date:  2021-03-26       Impact factor: 10.182
  5 in total

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