| Literature DB >> 17351616 |
Thomas Drepper1, Thorsten Eggert, Franco Circolone, Achim Heck, Ulrich Krauss, Jan-Karl Guterl, Marion Wendorff, Aba Losi, Wolfgang Gärtner, Karl-Erich Jaeger.
Abstract
Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real-time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue-light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems.Entities:
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Year: 2007 PMID： 17351616 DOI： 10.1038/nbt1293
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908