Literature DB >> 19376666

A simple 3-step algorithm for improved laboratory detection of Clostridium difficile toxin without the need for tissue culture cytotoxicity neutralization assays.

Susan E Sharp1, W Michael Ivie, Misti R Buckles, Diane M Coover, Julie C Pohl, Patricia A Hatcher.   

Abstract

This report describes a 3-step algorithm for the detection of Clostridium difficile using an enzyme immunoassay for glutamate dehydrogenase (GDH) antigen, a lateral flow assay for C. difficile toxin, and anaerobic broth culture. This method was found to detect more toxin-containing stool samples than the use of a combination GDH antigen plus toxin A/B testing alone.

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Year:  2009        PMID： 19376666     DOI： 10.1016/j.diagmicrobio.2009.03.009

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  7 in total

1.  A cost-effective approach for detection of toxigenic Clostridium difficile: toxigenic culture using ChromID Clostridium difficile agar.

Authors:  Shik Luk; Wing Kin To; Tak Keung Ng; Wai Ting Hui; Wing Keung Lee; Florence Lau; Almond Man Wai Ching
Journal:  J Clin Microbiol       Date:  2013-12-11       Impact factor: 5.948

2.  Evaluation of the C.Diff Quik Chek Complete Assay, a new glutamate dehydrogenase and A/B toxin combination lateral flow assay for use in rapid, simple diagnosis of clostridium difficile disease.

Authors:  Susan E Sharp; Lila O Ruden; Julie C Pohl; Patricia A Hatcher; Linda M Jayne; W Michael Ivie
Journal:  J Clin Microbiol       Date:  2010-04-07       Impact factor: 5.948

3.  Evaluation of a chromogenic culture medium for isolation of Clostridium difficile within 24 hours.

Authors:  John D Perry; Kerry Asir; Diane Halimi; Sylvain Orenga; Joanne Dale; Michelle Payne; Ruth Carlton; Jim Evans; F Kate Gould
Journal:  J Clin Microbiol       Date:  2010-08-25       Impact factor: 5.948

Review 4.  Diagnosis of Clostridium difficile infection: an ongoing conundrum for clinicians and for clinical laboratories.

Authors:  Carey-Ann D Burnham; Karen C Carroll
Journal:  Clin Microbiol Rev       Date:  2013-07       Impact factor: 26.132

5.  Evaluation of the Cepheid Xpert Clostridium difficile Epi assay for diagnosis of Clostridium difficile infection and typing of the NAP1 strain at a cancer hospital.

Authors:  N Esther Babady; Jeffrey Stiles; Phyllis Ruggiero; Perminder Khosa; David Huang; Susan Shuptar; Mini Kamboj; Timothy E Kiehn
Journal:  J Clin Microbiol       Date:  2010-10-13       Impact factor: 5.948

6.  Evolving concepts in Clostridium difficile colitis.

Authors:  Naomi G Diggs; Christina M Surawicz
Journal:  Curr Gastroenterol Rep       Date:  2009-10

7.  Multicenter evaluation of the Verigene Clostridium difficile nucleic acid assay.

Authors:  Karen C Carroll; Blake W Buchan; Sokha Tan; Paul D Stamper; Katherine M Riebe; Preeti Pancholi; Cheryl Kelly; Arundhati Rao; Robert Fader; Robert Cavagnolo; Wendy Watson; Richard V Goering; Ernest A Trevino; Alice S Weissfeld; Nathan A Ledeboer
Journal:  J Clin Microbiol       Date:  2013-10-02       Impact factor: 5.948
  7 in total

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