Literature DB >> 19360782

Tryptic digest analysis by comprehensive reversed phasextwo reversed phase liquid chromatography (RP-LCx2RP-LC) at different pH's.

Isabelle François1, Deirdre Cabooter, Koen Sandra, Frédéric Lynen, Gert Desmet, Pat Sandra.   

Abstract

As an alternative to the classical approach of combining strong cation exchange SCX-LC and RP-LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP-LCxRP-LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused-core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.

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Year:  2009        PMID： 19360782     DOI： 10.1002/jssc.200800578

Source DB:  PubMed          Journal:  J Sep Sci        ISSN： 1615-9306            Impact factor:   3.645


  8 in total

1.  Analysis of host-cell proteins in biotherapeutic proteins by comprehensive online two-dimensional liquid chromatography/mass spectrometry.

Authors:  Catalin E Doneanu; Alex Xenopoulos; Keith Fadgen; Jim Murphy; St John Skilton; Holly Prentice; Martha Stapels; Weibin Chen
Journal:  MAbs       Date:  2012 Jan-Feb       Impact factor: 5.857

2.  Characterization of an Ion Mobility-Multiplexed Collision Induced Dissociation-Tandem Time-of-Flight Mass Spectrometry Approach.

Authors:  Yehia M Ibrahim; David C Prior; Erin S Baker; Richard D Smith; Mikhail E Belov
Journal:  Int J Mass Spectrom       Date:  2010-06-01       Impact factor: 1.986

3.  An experimental study of sampling time effects on the resolving power of on-line two-dimensional high performance liquid chromatography.

Authors:  Yuan Huang; Haiwei Gu; Marcelo Filgueira; Peter W Carr
Journal:  J Chromatogr A       Date:  2011-04-12       Impact factor: 4.759

Review 4.  Multi-dimensional liquid chromatography in proteomics--a review.

Authors:  Xiang Zhang; Aiqin Fang; Catherine P Riley; Mu Wang; Fred E Regnier; Charles Buck
Journal:  Anal Chim Acta       Date:  2010-02-06       Impact factor: 6.558

5.  Increasing proteome coverage with offline RP HPLC coupled to online RP nanoLC-MS.

Authors:  Emine Gokce; Genna L Andrews; Ralph A Dean; David C Muddiman
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2011-02-04       Impact factor: 3.205

6.  Effects of first dimension eluent composition in two-dimensional liquid chromatography.

Authors:  Xiaoping Li; Peter W Carr
Journal:  J Chromatogr A       Date:  2011-02-16       Impact factor: 4.759

7.  High-pH reversed-phase chromatography with fraction concatenation for 2D proteomic analysis.

Authors:  Feng Yang; Yufeng Shen; David G Camp; Richard D Smith
Journal:  Expert Rev Proteomics       Date:  2012-04       Impact factor: 3.940

Review 8.  Advanced proteomic liquid chromatography.

Authors:  Fang Xie; Richard D Smith; Yufeng Shen
Journal:  J Chromatogr A       Date:  2012-07-09       Impact factor: 4.759
  8 in total

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