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Literature DB >> 19349979

Single molecule-sensitive probes for imaging RNA in live cells.

Philip J Santangelo¹, Aaron W Lifland, Paul Curt, Yukio Sasaki, Gary J Bassell, Michael E Lindquist, James E Crowe.

Abstract

To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.

Entities: CellLine Chemical Disease Gene Species

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Year: 2009 PMID： 19349979 PMCID： PMC4297622 DOI： 10.1038/nmeth.1316

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

14 in total

1. Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules.

Authors: Nancy Kedersha; Samantha Chen; Natalie Gilks; Wei Li; Ira J Miller; Joachim Stahl; Paul Anderson
Journal: Mol Biol Cell Date: 2002-01 Impact factor: 4.138

2. Mechanism of mRNA transport in the nucleus.

Authors: Diana Y Vargas; Arjun Raj; Salvatore A E Marras; Fred Russell Kramer; Sanjay Tyagi
Journal: Proc Natl Acad Sci U S A Date: 2005-11-11 Impact factor: 11.205

3. Translationally repressed mRNA transiently cycles through stress granules during stress.

Authors: Stephanie Mollet; Nicolas Cougot; Ania Wilczynska; François Dautry; Michel Kress; Edouard Bertrand; Dominique Weil
Journal: Mol Biol Cell Date: 2008-07-16 Impact factor: 4.138

4. Dynamic interaction between P-bodies and transport ribonucleoprotein particles in dendrites of mature hippocampal neurons.

Authors: Manuel Zeitelhofer; Daniela Karra; Paolo Macchi; Marco Tolino; Sabine Thomas; Martina Schwarz; Michael Kiebler; Ralf Dahm
Journal: J Neurosci Date: 2008-07-23 Impact factor: 6.167

Single molecule-sensitive probes for imaging RNA in live cells.

1. Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules.

2. Mechanism of mRNA transport in the nucleus.

3. Translationally repressed mRNA transiently cycles through stress granules during stress.

4. Dynamic interaction between P-bodies and transport ribonucleoprotein particles in dendrites of mature hippocampal neurons.

5. Visualization of single RNA transcripts in situ.

6. Respiratory syncytial virus uses a Vps4-independent budding mechanism controlled by Rab11-FIP2.

7. Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis.

8. Dynamic shuttling of TIA-1 accompanies the recruitment of mRNA to mammalian stress granules.

9. Stress granules and processing bodies are dynamically linked sites of mRNP remodeling.

10. Beta-actin mRNA localization is regulated by signal transduction mechanisms.

1. Dynamics of native β-actin mRNA transport in the cytoplasm.

2. Molecular-beacon-based tricomponent probe for SNP analysis in folded nucleic acids.

Review 3. DNA nanotechnology from the test tube to the cell.

Review 4. Imaging viral RNA using multiply labeled tetravalent RNA imaging probes in live cells.

5. Probes for intracellular RNA imaging in live cells.

6. Stress Granules and Virus Replication.

7. A Novel Method to Quantify RNA-Protein Interactions In Situ Using FMTRIP and Proximity Ligation.

Review 8. Teaching an Old Virus New Tricks: A Review on New Approaches to Study Age-Old Questions in Influenza Biology.

Review 9. Molecular mechanisms driving respiratory syncytial virus assembly.

Review 10. Advances in fluorescence labeling strategies for dynamic cellular imaging.