Literature DB >> 19331323

Molecular-scale force measurement in a coiled-coil peptide dimer by electron spin resonance.

Stefano V Gullà1, Gaurav Sharma, Peter Borbat, Jack H Freed, Harishchandra Ghimire, Monica R Benedikt, Natasha L Holt, Gary A Lorigan, Kaushal Rege, Constantinos Mavroidis, David E Budil.   

Abstract

A new method for measuring forces between small protein domains based on double electron-electron resonance (DEER) spectroscopy is demonstrated using a model peptide derived from the alpha-helical coiled-coil leucine zipper of yeast transcriptional activator GCN4. The equilibrium distribution of distances between two nitroxide spin labels rigidly attached to the helices of the dimer was determined by DEER and yielded a closing force of 100 +/- 10 pN between monomers, in excellent agreement with theoretical predictions.

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Year:  2009        PMID： 19331323      PMCID： PMC2888838          DOI： 10.1021/ja900230w

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  14 in total

1.  Engineering the morphology of a self-assembling protein fibre.

Authors:  Maxim G Ryadnov; Derek N Woolfson
Journal:  Nat Mater       Date:  2003-05       Impact factor: 43.841

2.  Synthesis and conformational studies of peptides containing TOAC, a spin-labelled C alpha, alpha-disubstituted glycine.

Authors:  C Toniolo; E Valente; F Formaggio; M Crisma; G Pilloni; C Corvaja; A Toffoletti; G V Martinez; M P Hanson; G L Millhauser
Journal:  J Pept Sci       Date:  1995 Jan-Feb       Impact factor: 1.905

3.  Scalable molecular dynamics with NAMD.

Authors:  James C Phillips; Rosemary Braun; Wei Wang; James Gumbart; Emad Tajkhorshid; Elizabeth Villa; Christophe Chipot; Robert D Skeel; Laxmikant Kalé; Klaus Schulten
Journal:  J Comput Chem       Date:  2005-12       Impact factor: 3.376

4.  Structure-based prediction of bZIP partnering specificity.

Authors:  Gevorg Grigoryan; Amy E Keating
Journal:  J Mol Biol       Date:  2005-12-01       Impact factor: 5.469

5.  The determination of pair distance distributions by pulsed ESR using Tikhonov regularization.

Authors:  Yun-Wei Chiang; Peter P Borbat; Jack H Freed
Journal:  J Magn Reson       Date:  2005-02       Impact factor: 2.229

Review 6.  Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy.

Authors:  Keir C Neuman; Attila Nagy
Journal:  Nat Methods       Date:  2008-06       Impact factor: 28.547

7.  MultiCoil: a program for predicting two- and three-stranded coiled coils.

Authors:  E Wolf; P S Kim; B Berger
Journal:  Protein Sci       Date:  1997-06       Impact factor: 6.725

8.  Predicting helix orientation for coiled-coil dimers.

Authors:  James R Apgar; Karl N Gutwin; Amy E Keating
Journal:  Proteins       Date:  2008-08-15

9.  Mapping backbone dynamics in solution with site-directed spin labeling: GCN4-58 bZip free and bound to DNA.

Authors:  Linda Columbus; Wayne L Hubbell
Journal:  Biochemistry       Date:  2004-06-15       Impact factor: 3.162

10.  Flexibility and lengths of bis-peptide nanostructures by electron spin resonance.

Authors:  Soraya Pornsuwan; Gregory Bird; Christian E Schafmeister; Sunil Saxena
Journal:  J Am Chem Soc       Date:  2006-03-29       Impact factor: 15.419
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  3 in total

Review 1.  From spin-labeled proteins to in vivo EPR applications.

Authors:  Lawrence J Berliner
Journal:  Eur Biophys J       Date:  2009-09-11       Impact factor: 1.733

2.  Significantly improved sensitivity of Q-band PELDOR/DEER experiments relative to X-band is observed in measuring the intercoil distance of a leucine zipper motif peptide (GCN4-LZ).

Authors:  Harishchandra Ghimire; Robert M McCarrick; David E Budil; Gary A Lorigan
Journal:  Biochemistry       Date:  2009-06-30       Impact factor: 3.162

3.  Improved Sensitivity for Long-Distance Measurements in Biomolecules: Five-Pulse Double Electron-Electron Resonance.

Authors:  Peter P Borbat; Elka R Georgieva; Jack H Freed
Journal:  J Phys Chem Lett       Date:  2012-12-19       Impact factor: 6.475
  3 in total

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