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Literature DB >> 19321720

Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica.

Shih-Yu Liang¹, Yun-Hsien Chan, Kan-Tai Hsia, Jing-Lun Lee, Ming-Chu Kuo, Kuo-Yuan Hwa, Chi-Wen Chan, Ting-Yi Chiang, Jung-Sheng Chen, Fang-Tzy Wu, Dar-Der Ji.

Abstract

A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.

Entities: Disease Species

Mesh：

Year: 2009 PMID： 19321720 PMCID： PMC2691116 DOI： 10.1128/JCM.00105-09

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

23 in total

1. [Proposals for innovative pharmacology education from the perspective of several fields--in response to rapidly changing times].

Authors: M Tanaka; M Misawa
Journal: Nihon Yakurigaku Zasshi Date: 2000-09

2. Loop-mediated isothermal amplification of DNA.

Authors: T Notomi; H Okayama; H Masubuchi; T Yonekawa; K Watanabe; N Amino; T Hase
Journal: Nucleic Acids Res Date: 2000-06-15 Impact factor: 16.971

3. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation.

Authors: Y Mori; K Nagamine; N Tomita; T Notomi
Journal: Biochem Biophys Res Commun Date: 2001-11-23 Impact factor: 3.575

4. Accelerated reaction by loop-mediated isothermal amplification using loop primers.

Authors: K Nagamine; T Hase; T Notomi
Journal: Mol Cell Probes Date: 2002-06 Impact factor: 2.365

5. Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis.

Authors: Andy Alhassan; Oriel M M Thekisoe; Naoaki Yokoyama; Noboru Inoue; Makhosazana Y Motloang; Peter A Mbati; Hong Yin; Yoshinari Katayama; Toru Anzai; Chihiro Sugimoto; Ikuo Igarashi
Journal: Vet Parasitol Date: 2006-09-14 Impact factor: 2.738

6. A nested, multiplex, PCR assay for the simultaneous detection and differentiation of Entamoeba histolytica and Entamoeba dispar in faeces.

Authors: A Evangelopoulos; G Spanakos; E Patsoula; N Vakalis; N Legakis
Journal: Ann Trop Med Parasitol Date: 2000-04

7. PCR detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA.

Authors: J L Holland; L Louie; A E Simor; M Louie
Journal: J Clin Microbiol Date: 2000-11 Impact factor: 5.948

8. Comparison of stool antigen detection kits to PCR for diagnosis of amebiasis.

Authors: D Stark; S van Hal; R Fotedar; A Butcher; D Marriott; J Ellis; J Harkness
Journal: J Clin Microbiol Date: 2008-03-26 Impact factor: 5.948

9. Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing.

Authors: Mohammed A Bakheit; Dena Torra; Lily A Palomino; Oriel M M Thekisoe; Peter A Mbati; Jerry Ongerth; Panagiotis Karanis
Journal: Vet Parasitol Date: 2008-09-11 Impact factor: 2.738

10. Detection of excretory Entamoeba histolytica DNA in the urine, and detection of E. histolytica DNA and lectin antigen in the liver abscess pus for the diagnosis of amoebic liver abscess.

Authors: Subhash C Parija; Krishna Khairnar
Journal: BMC Microbiol Date: 2007-05-18 Impact factor: 3.605

21 in total

1. Loop-mediated isothermal amplification (LAMP) assay for rapid detection of Entamoeba histolytica in amoebic liver abscess.

Authors: Preeti Singh; Bijay Ranjan Mirdha; Vineet Ahuja; Sundeep Singh
Journal: World J Microbiol Biotechnol Date: 2012-10-06 Impact factor: 3.312

2. Molecular test for vivax malaria with loop-mediated isothermal amplification method in central China.

Authors: Feng Lu; Qi Gao; Huayun Zhou; Jun Cao; Weimin Wang; Chae Seung Lim; SungHun Na; Takafumi Tsuboi; Eun-Taek Han
Journal: Parasitol Res Date: 2011-12-27 Impact factor: 2.289

3. Loop-mediated isothermal amplification as a reliable assay for Toxocara canis infection in pet dogs.

Authors: Paria Khoshakhlagh; Adel Spotin; Mahmoud Mahami-Oskouei; Abbas Shahbazi; Maryam Ozlati
Journal: Parasitol Res Date: 2017-07-09 Impact factor: 2.289

4. Development of a loop-mediated isothermal amplification assay for rapid detection of capripoxviruses.

Authors: Amaresh Das; Shawn Babiuk; Michael T McIntosh
Journal: J Clin Microbiol Date: 2012-02-22 Impact factor: 5.948

5. Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans.

Authors: Agathe Nkouawa; Yasuhito Sako; Tiaoying Li; Xingwang Chen; Toni Wandra; I Kadek Swastika; Minoru Nakao; Tetsuya Yanagida; Kazuhiro Nakaya; Dongchuan Qiu; Akira Ito
Journal: J Clin Microbiol Date: 2010-07-14 Impact factor: 5.948

Review 6. Update on laboratory diagnosis of amoebiasis.

Authors: Syazwan Saidin; Nurulhasanah Othman; Rahmah Noordin
Journal: Eur J Clin Microbiol Infect Dis Date: 2018-09-25 Impact factor: 3.267

7. Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions.

Authors: Jin-Long Yang; Su-Hui Zhang; Zuo-Hua Liu; Rui Yang; Yong Huang; Ming Wen
Journal: Virol J Date: 2012-12-29 Impact factor: 4.099