Literature DB >> 19274156

DNA sequences from formalin-fixed nematodes: integrating molecular and morphological approaches to taxonomy.

W K Thomas, J T Vida, L M Frisse, M Mundo, J G Baldwin.   

Abstract

To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological features. The fixed sections provided sufficient template for multiple polymerase chain reaction-based DNA sequence analyses.

Entities:  

Keywords:  DNA sequence; formalin; microdissection; molecular biology; nematode; polymerase chain reaction; taxonomy

Year:  1997        PMID： 19274156      PMCID： PMC2619797     

Source DB:  PubMed          Journal:  J Nematol        ISSN： 0022-300X            Impact factor:   1.402


  18 in total

1.  An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding.

Authors:  Paul De Ley; Irma Tandingan De Ley; Krystalynne Morris; Eyualem Abebe; Manuel Mundo-Ocampo; Melissa Yoder; Joseph Heras; Dora Waumann; Axayácatl Rocha-Olivares; A H Jay Burr; James G Baldwin; W Kelley Thomas
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2005-10-29       Impact factor: 6.237

2.  Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves.

Authors:  Lynn K Carta; Shiguang Li; Andrea M Skantar; George Newcombe
Journal:  J Nematol       Date:  2016-03       Impact factor: 1.402

3.  Improved 18S small subunit rDNA primers for problematic nematode amplification.

Authors:  L K Carta; S Li
Journal:  J Nematol       Date:  2018       Impact factor: 1.402

4.  Molecular rDNA phylogeny of Telotylenchidae Siddiqi, 1960 and evaluation of tail termini.

Authors:  L K Carta; A M Skantar; Z A Handoo
Journal:  J Nematol       Date:  2010-12       Impact factor: 1.402

5.  A trichodorus (triplonchida: trichodoridae) nematode from thrips (thysanoptera: panchaetothripinae).

Authors:  L K Carta; A M Skantar
Journal:  J Nematol       Date:  2014-09       Impact factor: 1.402

6.  A New Race (X12) of Soybean Cyst Nematode in China.

Authors:  Yun Lian; Jianqiu Guo; Haichao Li; Yongkang Wu; He Wei; Jinshe Wang; Jinying Li; Weiguo Lu
Journal:  J Nematol       Date:  2017-09       Impact factor: 1.402

7.  Molecular and Morphological Characterization of an Unusual Meloidogyne arenaria Population from Traveler's Tree, Ravenala madagascariensis.

Authors:  Am Skantar; Lk Carta; Za Handoo
Journal:  J Nematol       Date:  2008-09       Impact factor: 1.402

8.  Molecular and Morphological Characterization of the Corn Cyst Nematode, Heterodera zeae, from Greece.

Authors:  A M Skantar; Z A Handoo; G N Zanakis; E A Tzortzakakis
Journal:  J Nematol       Date:  2012-03       Impact factor: 1.402

9.  Description of Globodera ellingtonae n. sp. (Nematoda: Heteroderidae) from Oregon.

Authors:  Zafar A Handoo; Lynn K Carta; Andrea M Skantar; David J Chitwood
Journal:  J Nematol       Date:  2012-03       Impact factor: 1.402

10.  Molecular and morphological comparison of two different types of Habronema muscae (Nematoda: Habronematidae) in horse.

Authors:  Ehsan Rakhshandehroo; Hassan Sharifiyazdi; Hossein Shayegh; Amin Ahmadi
Journal:  Parasitol Res       Date:  2014-09-12       Impact factor: 2.289
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.