Literature DB >> 19270144

PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus subtilis spores.

H Rawsthorne1, C N Dock, L A Jaykus.   

Abstract

This paper describes a molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide. The approach should be valuable in our attempt to employ molecular methods to streamline the evaluation of process validation using bacterial endospores.

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Year:  2009        PMID： 19270144      PMCID： PMC2681676          DOI： 10.1128/AEM.02524-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Development of a real-time PCR assay targeting the sporulation gene, spo0A, for the enumeration of thermophilic bacilli in milk powder.

Authors:  Andreas Rueckert; Ron S Ronimus; Hugh W Morgan
Journal:  Food Microbiol       Date:  2005-07-05       Impact factor: 5.516

2.  Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide.

Authors:  Andreas Nocker; Anne K Camper
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792

3.  Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR.

Authors:  Shishan Wang; Robert E Levin
Journal:  J Microbiol Methods       Date:  2005-06-01       Impact factor: 2.363

4.  Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR.

Authors:  Hege Karin Nogva; Signe Marie Drømtorp; Hilde Nissen; Knut Rudi
Journal:  Biotechniques       Date:  2003-04       Impact factor: 1.993

5.  Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples.

Authors:  Knut Rudi; Birgitte Moen; Signe Marie Drømtorp; Askild L Holck
Journal:  Appl Environ Microbiol       Date:  2005-02       Impact factor: 4.792

6.  Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR.

Authors:  K Rudi; K Naterstad; S M Drømtorp; H Holo
Journal:  Lett Appl Microbiol       Date:  2005       Impact factor: 2.858

7.  Thermal destruction of Clostridium botulinum spores suspended in tomato juice in aluminum thermal death time tubes.

Authors:  T E Odlaug; I J Pflug
Journal:  Appl Environ Microbiol       Date:  1977-07       Impact factor: 4.792
  7 in total
  24 in total

Review 1.  Next-generation sequencing in the analysis of human microbiota: essential considerations for clinical application.

Authors:  Geraint B Rogers; Kenneth D Bruce
Journal:  Mol Diagn Ther       Date:  2010-12-01       Impact factor: 4.074

2.  Assessment of Legionella pneumophila in recreational spring water with quantitative PCR (Taqman) assay.

Authors:  Shu-Min Shen; Ming-Yuan Chou; Bing-Mu Hsu; Wen-Tsai Ji; Tsui-Kang Hsu; Hsiu-Feng Tsai; Yu-Li Huang; Yi-Chou Chiu; Erl-Shyh Kao; Po-Min Kao; Cheng-Wei Fan
Journal:  Pathog Glob Health       Date:  2015-07-16       Impact factor: 2.894

Review 3.  Dead or alive: molecular assessment of microbial viability.

Authors:  Gerard A Cangelosi; John S Meschke
Journal:  Appl Environ Microbiol       Date:  2014-07-18       Impact factor: 4.792

4.  New perspectives on viable microbial communities in low-biomass cleanroom environments.

Authors:  Parag Vaishampayan; Alexander J Probst; Myron T La Duc; Emilee Bargoma; James N Benardini; Gary L Andersen; Kasthuri Venkateswaran
Journal:  ISME J       Date:  2012-10-11       Impact factor: 10.302

5.  Selective quantification of viable Escherichia coli bacteria in biosolids by quantitative PCR with propidium monoazide modification.

Authors:  Bilgin Taskin; Ayse Gul Gozen; Metin Duran
Journal:  Appl Environ Microbiol       Date:  2011-05-20       Impact factor: 4.792

6.  Validation of a Clostridium endospore viability assay and analysis of Greenland ices and Atacama Desert soils.

Authors:  Wan-Wan Yang; Adrian Ponce
Journal:  Appl Environ Microbiol       Date:  2011-02-04       Impact factor: 4.792

7.  Health risk from the use of roof-harvested rainwater in Southeast Queensland, Australia, as potable or nonpotable water, determined using quantitative microbial risk assessment.

Authors:  W Ahmed; A Vieritz; A Goonetilleke; T Gardner
Journal:  Appl Environ Microbiol       Date:  2010-09-17       Impact factor: 4.792

8.  Evaluation of propidium monoazide-based qPCR to detect viable oocysts of Toxoplasma gondii.

Authors:  Angélique Rousseau; Isabelle Villena; Aurélien Dumètre; Sandie Escotte-Binet; Loïc Favennec; Jitender P Dubey; Dominique Aubert; Stéphanie La Carbona
Journal:  Parasitol Res       Date:  2019-02-07       Impact factor: 2.289

9.  Viable real-time PCR in environmental samples: can all data be interpreted directly?

Authors:  Mariana Fittipaldi; Francesc Codony; Barbara Adrados; Anne K Camper; Jordi Morató
Journal:  Microb Ecol       Date:  2010-07-15       Impact factor: 4.552

10.  Detecting the nonviable and heat-tolerant bacteria in activated sludge by minimizing DNA from dead cells.

Authors:  Feng Guo; Tong Zhang
Journal:  Microb Ecol       Date:  2014-02-18       Impact factor: 4.552
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