| Literature DB >> 19268654 |
Li Li1, Daxin Pang, Tiedong Wang, Zhanjun Li, Limei Chen, Mingjun Zhang, Na Song, Daibang Nie, Zhenwen Chen, Liangxue Lai, Hongsheng Ouyang.
Abstract
The pig is thought to be the most suitable non-human source of organs for xenotransplantation and is widely used as a model of human disease. Using pigs as disease models requires the design of conditional Cre recombinase-loxP gene modifications, which, in turn, requires a Cre-expressing pig with defined patterns of expression controlled by the use of a tissue-specific promoter. In order to monitor Cre recombinant expression in vivo, it is important to create a reporter strain. We have generated reporter a pig that is based on a single vector that drives the ubiquitous expression of the enhanced green fluorescent protein (EGFP). The EGFP gene is expressed only after Cre-mediated excision of loxP-flanked stop sequences. These reporter transgenic pigs will be of great value for monitoring Cre recombinase activity in vivo.Entities:
Mesh：
Substances：
Year: 2009 PMID： 19268654 DOI： 10.1016/j.bbrc.2009.02.146
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575