Literature DB >> 19232863

Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates.

Carson D Baldwin1, Gerald B Howe, Ranga Sampath, Larry B Blyn, Heather Matthews, Vanessa Harpin, Thomas A Hall, Jared J Drader, Steve A Hofstadler, Mark W Eshoo, Karl Rudnick, Karen Studarus, David Moore, Sharon Abbott, J Michael Janda, Chris A Whitehouse.   

Abstract

Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.

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Year:  2009        PMID： 19232863     DOI： 10.1016/j.diagmicrobio.2008.12.012

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  19 in total

1.  Identification of pathogenic Vibrio species by multilocus PCR-electrospray ionization mass spectrometry and its application to aquatic environments of the former soviet republic of Georgia.

Authors:  Chris A Whitehouse; Carson Baldwin; Rangarajan Sampath; Lawrence B Blyn; Rachael Melton; Feng Li; Thomas A Hall; Vanessa Harpin; Heather Matthews; Marina Tediashvili; Ekaterina Jaiani; Tamar Kokashvili; Nino Janelidze; Christopher Grim; Rita R Colwell; Anwar Huq
Journal:  Appl Environ Microbiol       Date:  2010-01-29       Impact factor: 4.792

2.  Broad-spectrum biosensor capable of detecting and identifying diverse bacterial and Candida species in blood.

Authors:  David Metzgar; Mark Frinder; Robert Lovari; Donna Toleno; Christian Massire; Lawrence B Blyn; Raymond Ranken; Heather E Carolan; Thomas A Hall; David Moore; Christian J Hansen; Rangarajan Sampath; David J Ecker
Journal:  J Clin Microbiol       Date:  2013-06-12       Impact factor: 5.948

3.  Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles.

Authors:  Erin J Kaleta; Andrew E Clark; Desiree R Johnson; Dulini C Gamage; Vicki H Wysocki; Abdessalam Cherkaoui; Jacques Schrenzel; Donna M Wolk
Journal:  J Clin Microbiol       Date:  2010-11-03       Impact factor: 5.948

4.  Rapid universal identification of bacterial pathogens from clinical cultures by using a novel sloppy molecular beacon melting temperature signature technique.

Authors:  Soumitesh Chakravorty; Bola Aladegbami; Michele Burday; Michael Levi; Salvatore A E Marras; Darshini Shah; Hiyam H El-Hajj; Fred Russell Kramer; David Alland
Journal:  J Clin Microbiol       Date:  2009-11-18       Impact factor: 5.948

5.  Detection, identification, and distribution of fungi in bronchoalveolar lavage specimens by use of multilocus PCR coupled with electrospray ionization/mass spectrometry.

Authors:  Jeong Hwan Shin; Raymond Ranken; Susan E Sefers; Robert Lovari; Criziel D Quinn; Shufang Meng; Heather E Carolan; Donna Toleno; Haijing Li; Jeong Nyeo Lee; Charles W Stratton; Christian Massire; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2012-10-24       Impact factor: 5.948

6.  Detection of prosthetic joint infection by use of PCR-electrospray ionization mass spectrometry applied to synovial fluid.

Authors:  Dante P Melendez; James R Uhl; Kerryl E Greenwood-Quaintance; Arlen D Hanssen; Rangarajan Sampath; Robin Patel
Journal:  J Clin Microbiol       Date:  2014-04-09       Impact factor: 5.948

Review 7.  Expansion of Microbial Forensics.

Authors:  Sarah E Schmedes; Antti Sajantila; Bruce Budowle
Journal:  J Clin Microbiol       Date:  2016-02-24       Impact factor: 5.948

8.  Informing Antibiotic Treatment Decisions: Evaluating Rapid Molecular Diagnostics To Identify Susceptibility and Resistance to Carbapenems against Acinetobacter spp. in PRIMERS III.

Authors:  Scott R Evans; Andrea M Hujer; Hongyu Jiang; Carol B Hill; Kristine M Hujer; Jose R Mediavilla; Claudia Manca; Thuy Tien T Tran; T Nicholas Domitrovic; Paul G Higgins; Harald Seifert; Barry N Kreiswirth; Robin Patel; Michael R Jacobs; Liang Chen; Rangarajan Sampath; Thomas Hall; Christine Marzan; Vance G Fowler; Henry F Chambers; Robert A Bonomo
Journal:  J Clin Microbiol       Date:  2016-12-28       Impact factor: 5.948

9.  Extraction of total nucleic acids from ticks for the detection of bacterial and viral pathogens.

Authors:  Chris D Crowder; Megan A Rounds; Curtis A Phillipson; John M Picuri; Heather E Matthews; Justina Halverson; Steven E Schutzer; David J Ecker; Mark W Eshoo
Journal:  J Med Entomol       Date:  2010-01       Impact factor: 2.278

10.  PCR-electrospray ionization mass spectrometry for direct detection of pathogens and antimicrobial resistance from heart valves in patients with infective endocarditis.

Authors:  Cassandra L Brinkman; Paschalis Vergidis; James R Uhl; Bobbi S Pritt; Franklin R Cockerill; James M Steckelberg; Larry M Baddour; Joseph J Maleszewski; William D Edwards; Rangarajan Sampath; Robin Patel
Journal:  J Clin Microbiol       Date:  2013-04-17       Impact factor: 5.948
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