Literature DB >> 19209332

Computer-enhanced high-throughput genetic screens of C. elegans in a microfluidic system.

Matthew M Crane1, Kwanghun Chung, Hang Lu.   

Abstract

Most visual screens based on fluorescent markers are currently limited in throughput and accuracy. Here we present the first genetic screen of a mutagenized population of C. elegans in a microfluidic device. Animal handling streamlined by a microfluidic device and intuitive control software enabled the identification of novel mutants and a large screening speed.

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Year:  2008        PMID： 19209332     DOI： 10.1039/b813730g

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  32 in total

1.  High-throughput study of synaptic transmission at the neuromuscular junction enabled by optogenetics and microfluidics.

Authors:  Jeffrey N Stirman; Martin Brauner; Alexander Gottschalk; Hang Lu
Journal:  J Neurosci Methods       Date:  2010-06-09       Impact factor: 2.390

2.  A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans.

Authors:  Shawn R Lockery; S Elizabeth Hulme; William M Roberts; Kristin J Robinson; Anna Laromaine; Theodore H Lindsay; George M Whitesides; Janis C Weeks
Journal:  Lab Chip       Date:  2012-05-15       Impact factor: 6.799

3.  The impact of whole genome sequencing on model system genetics: get ready for the ride.

Authors:  Oliver Hobert
Journal:  Genetics       Date:  2010-02       Impact factor: 4.562

4.  A perspective on optical developments in microfluidic platforms for Caenorhabditis elegans research.

Authors:  Guillaume Aubry; Hang Lu
Journal:  Biomicrofluidics       Date:  2014-02-13       Impact factor: 2.800

Review 5.  Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans.

Authors:  Maria Doitsidou; Sophie Jarriault; Richard J Poole
Journal:  Genetics       Date:  2016-10       Impact factor: 4.562

Review 6.  Microfluidic tools for developmental studies of small model organisms--nematodes, fruit flies, and zebrafish.

Authors:  Hyundoo Hwang; Hang Lu
Journal:  Biotechnol J       Date:  2012-11-19       Impact factor: 4.677

7.  Microfluidic trap array for massively parallel imaging of Drosophila embryos.

Authors:  Thomas J Levario; Mei Zhan; Bomyi Lim; Stanislav Y Shvartsman; Hang Lu
Journal:  Nat Protoc       Date:  2013-03-14       Impact factor: 13.491

8.  A multi-channel device for high-density target-selective stimulation and long-term monitoring of cells and subcellular features in C. elegans.

Authors:  Hyewon Lee; Shin Ae Kim; Sean Coakley; Paula Mugno; Marc Hammarlund; Massimo A Hilliard; Hang Lu
Journal:  Lab Chip       Date:  2014-09-26       Impact factor: 6.799

9.  A programmable microvalve-based microfluidic array for characterization of neurotoxin-induced responses of individual C. elegans.

Authors:  Hui Ma; Lei Jiang; Weiwei Shi; Jianhua Qin; Bingcheng Lin
Journal:  Biomicrofluidics       Date:  2009-12-23       Impact factor: 2.800

10.  Fluorescence-based sorting of Caenorhabditis elegans via acoustofluidics.

Authors:  Jinxin Zhang; Jessica H Hartman; Chuyi Chen; Shujie Yang; Qi Li; Zhenhua Tian; Po-Hsun Huang; Lin Wang; Joel N Meyer; Tony Jun Huang
Journal:  Lab Chip       Date:  2020-05-19       Impact factor: 6.799
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