OBJECTIVE: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). DESIGN AND METHODS: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n=150, age: 46.6+/-7.7 years), and female controls (n=150, age: 46.8+/-7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. RESULTS: Levels of modified nucleosides (5-hydroxymethyl-2'-deoxyuridine, P<0.001; 8-hydroxy-2'-deoxyguanosine, P<0.001; 1-methyladenosine, P<0.02; N(2),N(2)-dimethylguanosine, P<0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. CONCLUSIONS: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.
OBJECTIVE: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). DESIGN AND METHODS: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n=150, age: 46.6+/-7.7 years), and female controls (n=150, age: 46.8+/-7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. RESULTS: Levels of modified nucleosides (5-hydroxymethyl-2'-deoxyuridine, P<0.001; 8-hydroxy-2'-deoxyguanosine, P<0.001; 1-methyladenosine, P<0.02; N(2),N(2)-dimethylguanosine, P<0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. CONCLUSIONS: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.
Authors: S Dulong; Q Huang; P F Innominato; A Karaboue; M Bouchahda; A Pruvost; F Théodoro; L A Agrofoglio; R Adam; B Finkenstädt; F Lévi Journal: Sci Rep Date: 2021-12-14 Impact factor: 4.379