Literature DB >> 19177520

Parallel screening and optimization of protein constructs for structural studies.

Rodolfo M Rasia1, Marjolaine Noirclerc-Savoye, Nicolás G Bologna, Benoit Gallet, Michael J Plevin, Laurence Blanchard, Javier F Palatnik, Bernhard Brutscher, Thierry Vernet, Jérôme Boisbouvier.   

Abstract

A major challenge in structural biology remains the identification of protein constructs amenable to structural characterization. Here, we present a simple method for parallel expression, labeling, and purification of protein constructs (up to 80 kDa) combined with rapid evaluation by NMR spectroscopy. Our approach, which is equally applicable for manual or automated implementation, offers an efficient way to identify and optimize protein constructs for NMR or X-ray crystallographic investigations.

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Year:  2009        PMID： 19177520      PMCID： PMC2708052          DOI： 10.1002/pro.46

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  22 in total

1.  An NMR approach to structural proteomics.

Authors:  Adelinda Yee; Xiaoqing Chang; Antonio Pineda-Lucena; Bin Wu; Anthony Semesi; Brian Le; Theresa Ramelot; Gregory M Lee; Sudeepa Bhattacharyya; Pablo Gutierrez; Aleksej Denisov; Chang-Hun Lee; John R Cort; Guennadi Kozlov; Jack Liao; Grzegorz Finak; Limin Chen; David Wishart; Weontae Lee; Lawrence P McIntosh; Kalle Gehring; Michael A Kennedy; Aled M Edwards; Cheryl H Arrowsmith
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-19       Impact factor: 11.205

2.  Screening methods to determine biophysical properties of proteins in structural genomics.

Authors:  Esmeralda A Woestenenk; Martin Hammarström; Torleif Härd; Helena Berglund
Journal:  Anal Biochem       Date:  2003-07-01       Impact factor: 3.365

3.  Expression screening, protein purification and NMR analysis of human protein domains for structural genomics.

Authors:  G E Folkers; B N M van Buuren; R Kaptein
Journal:  J Struct Funct Genomics       Date:  2004

4.  Rapid screening for structural integrity of expressed proteins by heteronuclear NMR spectroscopy.

Authors:  A M Gronenborn; G M Clore
Journal:  Protein Sci       Date:  1996-01       Impact factor: 6.725

5.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

6.  Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution.

Authors:  K Pervushin; R Riek; G Wider; K Wüthrich
Journal:  Proc Natl Acad Sci U S A       Date:  1997-11-11       Impact factor: 11.205

7.  In situ proteolysis for protein crystallization and structure determination.

Authors:  Aiping Dong; Xiaohui Xu; Aled M Edwards; Changsoo Chang; Maksymilian Chruszcz; Marianne Cuff; Marcin Cymborowski; Rosa Di Leo; Olga Egorova; Elena Evdokimova; Ekaterina Filippova; Jun Gu; Jennifer Guthrie; Alexandr Ignatchenko; Andrzej Joachimiak; Natalie Klostermann; Youngchang Kim; Yuri Korniyenko; Wladek Minor; Qiuni Que; Alexei Savchenko; Tatiana Skarina; Kemin Tan; Alexander Yakunin; Adelinda Yee; Veronica Yim; Rongguang Zhang; Hong Zheng; Masato Akutsu; Cheryl Arrowsmith; George V Avvakumov; Alexey Bochkarev; Lars-Göran Dahlgren; Sirano Dhe-Paganon; Slav Dimov; Ludmila Dombrovski; Patrick Finerty; Susanne Flodin; Alex Flores; Susanne Gräslund; Martin Hammerström; Maria Dolores Herman; Bum-Soo Hong; Raymond Hui; Ida Johansson; Yongson Liu; Martina Nilsson; Lyudmila Nedyalkova; Pär Nordlund; Tomas Nyman; Jinrong Min; Hui Ouyang; Hee-won Park; Chao Qi; Wael Rabeh; Limin Shen; Yang Shen; Deepthi Sukumard; Wolfram Tempel; Yufeng Tong; Lionel Tresagues; Masoud Vedadi; John R Walker; Johan Weigelt; Martin Welin; Hong Wu; Ting Xiao; Hong Zeng; Haizhong Zhu
Journal:  Nat Methods       Date:  2007-11-04       Impact factor: 28.547

8.  The nuclear dsRNA binding protein HYL1 is required for microRNA accumulation and plant development, but not posttranscriptional transgene silencing.

Authors:  Franck Vazquez; Virginie Gasciolli; Patrice Crété; Hervé Vaucheret
Journal:  Curr Biol       Date:  2004-02-17       Impact factor: 10.834

9.  The Arabidopsis double-stranded RNA-binding protein HYL1 plays a role in microRNA-mediated gene regulation.

Authors:  Meng-Hsuan Han; Saiprasad Goud; Liang Song; Nina Fedoroff
Journal:  Proc Natl Acad Sci U S A       Date:  2004-01-13       Impact factor: 11.205

10.  Refolding out of guanidine hydrochloride is an effective approach for high-throughput structural studies of small proteins.

Authors:  Karen L Maxwell; Diane Bona; Chengsong Liu; Cheryl H Arrowsmith; Aled M Edwards
Journal:  Protein Sci       Date:  2003-09       Impact factor: 6.725
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  1 in total

1.  A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.

Authors:  Carlos Amero; M Asunción Durá; Marjolaine Noirclerc-Savoye; Arnaud Perollier; Benoit Gallet; Michael J Plevin; Thierry Vernet; Bruno Franzetti; Jérôme Boisbouvier
Journal:  J Biomol NMR       Date:  2011-05-29       Impact factor: 2.835
  1 in total

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