Literature DB >> 19173359

Fast biosynthesis of GFP molecules: a single-molecule fluorescence study.

Alexandros Katranidis1, Diaa Atta, Ramona Schlesinger, Knud H Nierhaus, Theodora Choli-Papadopoulou, Ingo Gregor, Michael Gerrits, Georg Büldt, Jörg Fitter.   

Abstract

It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute (see picture).

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Year:  2009        PMID： 19173359     DOI： 10.1002/anie.200806070

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  14 in total

1.  Translation and folding of single proteins in real time.

Authors:  Florian Wruck; Alexandros Katranidis; Knud H Nierhaus; Georg Büldt; Martin Hegner
Journal:  Proc Natl Acad Sci U S A       Date:  2017-05-15       Impact factor: 11.205

2.  On the GFP-based analysis of dynamic concentration profiles.

Authors:  Alexander M Berezhkovskii; Stanislav Y Shvartsman
Journal:  Biophys J       Date:  2014-02-04       Impact factor: 4.033

Review 3.  In vitro and in vivo single-molecule fluorescence imaging of ribosome-catalyzed protein synthesis.

Authors:  Corey E Perez; Ruben L Gonzalez
Journal:  Curr Opin Chem Biol       Date:  2011-11-19       Impact factor: 8.822

4.  Quantifying elongation rhythm during full-length protein synthesis.

Authors:  Gabriel Rosenblum; Chunlai Chen; Jaskiran Kaur; Xiaonan Cui; Haibo Zhang; Haruichi Asahara; Shaorong Chong; Zeev Smilansky; Yale E Goldman; Barry S Cooperman
Journal:  J Am Chem Soc       Date:  2013-07-16       Impact factor: 15.419

Review 5.  Protein folding at single-molecule resolution.

Authors:  Allan Chris M Ferreon; Ashok A Deniz
Journal:  Biochim Biophys Acta       Date:  2011-02-17

6.  Chromophore maturation and fluorescence fluctuation spectroscopy of fluorescent proteins in a cell-free expression system.

Authors:  Patrick J Macdonald; Yan Chen; Joachim D Mueller
Journal:  Anal Biochem       Date:  2011-10-28       Impact factor: 3.365

Review 7.  Engine out of the chassis: cell-free protein synthesis and its uses.

Authors:  Gabriel Rosenblum; Barry S Cooperman
Journal:  FEBS Lett       Date:  2013-10-22       Impact factor: 4.124

8.  Real-time assay for testing components of protein synthesis.

Authors:  Gabriel Rosenblum; Chunlai Chen; Jaskiran Kaur; Xiaonan Cui; Yale E Goldman; Barry S Cooperman
Journal:  Nucleic Acids Res       Date:  2012-03-14       Impact factor: 16.971

9.  Mechanistic Insight into the Reactivation of BCAII Enzyme from Denatured and Molten Globule States by Eukaryotic Ribosomes and Domain V rRNAs.

Authors:  Biprashekhar Chakraborty; Sayan Bhakta; Jayati Sengupta
Journal:  PLoS One       Date:  2016-04-21       Impact factor: 3.240

10.  A Novel Method to Evaluate Ribosomal Performance in Cell-Free Protein Synthesis Systems.

Authors:  Noémie Kempf; Cristina Remes; Ralph Ledesch; Tina Züchner; Henning Höfig; Ilona Ritter; Alexandros Katranidis; Jörg Fitter
Journal:  Sci Rep       Date:  2017-04-24       Impact factor: 4.379
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