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Literature DB >> 19142923

Compartmentalization of chemically separated components into droplets.

J Scott Edgar¹, Graham Milne, Yiqiong Zhao, Chaitanya P Pabbati, David S W Lim, Daniel T Chiu.

Abstract

Not merely a drop in the ocean: The integration of capillary electrophoresis (CE) with droplet generation driven by electroosmotic flow enabled the compartimentalization of molecular components separated by CE in a series of droplets (see picture; the green bars represent the separated analytes). The droplet-confined bands can be docked and studied on a chip.

Entities: Chemical Gene

Mesh：

Year: 2009 PMID： 19142923 PMCID： PMC2865894 DOI： 10.1002/anie.200805396

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

19 in total

1. Monodisperse double emulsions generated from a microcapillary device.

Authors: A S Utada; E Lorenceau; D R Link; P D Kaplan; H A Stone; D A Weitz
Journal: Science Date: 2005-04-22 Impact factor: 47.728

2. Microfluidic flow focusing: drop size and scaling in pressure versus flow-rate-driven pumping.

Authors: Thomas Ward; Magalie Faivre; Manouk Abkarian; Howard A Stone
Journal: Electrophoresis Date: 2005-10 Impact factor: 3.535

3. Counting low-copy number proteins in a single cell.

Authors: Bo Huang; Hongkai Wu; Devaki Bhaya; Arthur Grossman; Sebastien Granier; Brian K Kobilka; Richard N Zare
Journal: Science Date: 2007-01-05 Impact factor: 47.728

4. Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis.

Authors: J Scott Edgar; Chaitanya P Pabbati; Robert M Lorenz; Mingyan He; Gina S Fiorini; Daniel T Chiu
Journal: Anal Chem Date: 2006-10-01 Impact factor: 6.986

Review 5. Reactions in droplets in microfluidic channels.

Authors: Helen Song; Delai L Chen; Rustem F Ismagilov
Journal: Angew Chem Int Ed Engl Date: 2006-11-13 Impact factor: 15.336

6. Microfluidic separation and capture of analytes for single-molecule spectroscopy.

Authors: Samuel Kim; Bo Huang; Richard N Zare
Journal: Lab Chip Date: 2007-10-23 Impact factor: 6.799

7. Continuous-flow single-molecule CE with high detection efficiency.

Authors: Perry G Schiro; Christopher L Kuyper; Daniel T Chiu
Journal: Electrophoresis Date: 2007-07 Impact factor: 3.535

8. Droplet-based microfluidic system for individual Caenorhabditis elegans assay.

Authors: Weiwei Shi; Jianhua Qin; Nannan Ye; Bingcheng Lin
Journal: Lab Chip Date: 2008-07-07 Impact factor: 6.799

9. Imaging of Pressure- and Electrokinetically Driven Flows through Open Capillaries.

Authors: P H Paul; M G Garguilo; D J Rakestraw
Journal: Anal Chem Date: 1998-07-01 Impact factor: 6.986

10. Sampling and electrophoretic analysis of segmented flow streams using virtual walls in a microfluidic device.

Authors: Gregory T Roman; Meng Wang; Kristin N Shultz; Colin Jennings; Robert T Kennedy
Journal: Anal Chem Date: 2008-10-03 Impact factor: 6.986

20 in total

1. High-resolution dose-response screening using droplet-based microfluidics.

Authors: Oliver J Miller; Abdeslam El Harrak; Thomas Mangeat; Jean-Christophe Baret; Lucas Frenz; Bachir El Debs; Estelle Mayot; Michael L Samuels; Eamonn K Rooney; Pierre Dieu; Martin Galvan; Darren R Link; Andrew D Griffiths
Journal: Proc Natl Acad Sci U S A Date: 2011-12-27 Impact factor: 11.205

Compartmentalization of chemically separated components into droplets.

1. Monodisperse double emulsions generated from a microcapillary device.

2. Microfluidic flow focusing: drop size and scaling in pressure versus flow-rate-driven pumping.

3. Counting low-copy number proteins in a single cell.

4. Capillary electrophoresis separation in the presence of an immiscible boundary for droplet analysis.

Review 5. Reactions in droplets in microfluidic channels.

6. Microfluidic separation and capture of analytes for single-molecule spectroscopy.

7. Continuous-flow single-molecule CE with high detection efficiency.

8. Droplet-based microfluidic system for individual Caenorhabditis elegans assay.

9. Imaging of Pressure- and Electrokinetically Driven Flows through Open Capillaries.

10. Sampling and electrophoretic analysis of segmented flow streams using virtual walls in a microfluidic device.

1. High-resolution dose-response screening using droplet-based microfluidics.

2. The potential impact of droplet microfluidics in biology.

Review 3. Advances in microfluidic materials, functions, integration, and applications.

4. Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.

5. A microfluidic chip for ICPMS sample introduction.

Review 6. Controlling mass transport in microfluidic devices.

7. Droplet freezing, docking, and the exchange of immiscible phase and surfactant around frozen droplets.

8. Self-digitization of samples into a high-density microfluidic bottom-well array.

9. Droplets for ultrasmall-volume analysis.

10. Dilution-free analysis from picoliter droplets by nano-electrospray ionization mass spectrometry.