Literature DB >> 21671557

Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.

Chia-Hung Chen1, Aniruddh Sarkar, Yong-Ak Song, Miles A Miller, Sung Jae Kim, Linda G Griffith, Douglas A Lauffenburger, Jongyoon Han.   

Abstract

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.

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Year:  2011        PMID： 21671557      PMCID： PMC3165005          DOI： 10.1021/ja2036628

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  41 in total

1.  High-throughput injection with microfluidics using picoinjectors.

Authors:  Adam R Abate; Tony Hung; Pascaline Mary; Jeremy J Agresti; David A Weitz
Journal:  Proc Natl Acad Sci U S A       Date:  2010-10-20       Impact factor: 11.205

2.  Proteolytic Activity Matrix Analysis (PrAMA) for simultaneous determination of multiple protease activities.

Authors:  Miles A Miller; Layla Barkal; Karen Jeng; Andreas Herrlich; Marcia Moss; Linda G Griffith; Douglas A Lauffenburger
Journal:  Integr Biol (Camb)       Date:  2010-12-23       Impact factor: 2.192

3.  Dynamic imaging of protease activity with fluorescently quenched activity-based probes.

Authors:  Galia Blum; Stefanie R Mullins; Kinneret Keren; Marko Fonovic; Christopher Jedeszko; Mark J Rice; Bonnie F Sloane; Matthew Bogyo
Journal:  Nat Chem Biol       Date:  2005-08-14       Impact factor: 15.040

Review 4.  Reactions in droplets in microfluidic channels.

Authors:  Helen Song; Delai L Chen; Rustem F Ismagilov
Journal:  Angew Chem Int Ed Engl       Date:  2006-11-13       Impact factor: 15.336

5.  Concentration polarization and nonlinear electrokinetic flow near a nanofluidic channel.

Authors:  Sung Jae Kim; Ying-Chih Wang; Jeong Hoon Lee; Hongchul Jang; Jongyoon Han
Journal:  Phys Rev Lett       Date:  2007-07-25       Impact factor: 9.161

6.  Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane.

Authors:  Jeong Hoon Lee; Yong-Ak Song; Jongyoon Han
Journal:  Lab Chip       Date:  2008-03-04       Impact factor: 6.799

7.  Quantitative reverse zymography: analysis of picogram amounts of metalloproteinase inhibitors using gelatinase A and B reverse zymograms.

Authors:  G W Oliver; J D Leferson; W G Stetler-Stevenson; D E Kleiner
Journal:  Anal Biochem       Date:  1997-01-01       Impact factor: 3.365

8.  Direct seawater desalination by ion concentration polarization.

Authors:  Sung Jae Kim; Sung Hee Ko; Kwan Hyoung Kang; Jongyoon Han
Journal:  Nat Nanotechnol       Date:  2010-03-21       Impact factor: 39.213

9.  Carcinoma and stromal enzyme activity profiles associated with breast tumor growth in vivo.

Authors:  Nadim Jessani; Mark Humphrey; W Hayes McDonald; Sherry Niessen; Kim Masuda; Beena Gangadharan; John R Yates; Barbara M Mueller; Benjamin F Cravatt
Journal:  Proc Natl Acad Sci U S A       Date:  2004-09-08       Impact factor: 11.205

10.  Laterally mobile, functionalized self-assembled monolayers at the fluorous-aqueous interface in a plug-based microfluidic system: characterization and testing with membrane protein crystallization.

Authors:  Jason E Kreutz; Liang Li; L Spencer Roach; Takuji Hatakeyama; Rustem F Ismagilov
Journal:  J Am Chem Soc       Date:  2009-05-06       Impact factor: 15.419
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  16 in total

1.  Capillarity ion concentration polarization for spontaneous biomolecular preconcentration mechanism.

Authors:  Yoonjee Oh; Hyomin Lee; Seok Young Son; Sung Jae Kim; Pilnam Kim
Journal:  Biomicrofluidics       Date:  2016-01-07       Impact factor: 2.800

Review 2.  Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.

Authors:  Aniruddha M Kaushik; Kuangwen Hsieh; Tza-Huei Wang
Journal:  Wiley Interdiscip Rev Nanomed Nanobiotechnol       Date:  2018-05-24

3.  Nanoelectrokinetic bufferchannel-less radial preconcentrator and online extractor by tunable ion depletion layer.

Authors:  Sangjun Lee; Sungmin Park; Wonseok Kim; Suhong Moon; Ho-Young Kim; Hyomin Lee; Sung Jae Kim
Journal:  Biomicrofluidics       Date:  2019-05-30       Impact factor: 2.800

4.  Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone.

Authors:  Minyoung Kim; Hyunjoon Rhee; Ji Yoon Kang; Tae Song Kim; Rhokyun Kwak
Journal:  J Vis Exp       Date:  2017-02-23       Impact factor: 1.355

Review 5.  Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.

Authors:  Michelle L Kovarik; Douglas M Ornoff; Adam T Melvin; Nicholas C Dobes; Yuli Wang; Alexandra J Dickinson; Philip C Gach; Pavak K Shah; Nancy L Allbritton
Journal:  Anal Chem       Date:  2012-12-04       Impact factor: 6.986

6.  Multivesicular droplets: a cell model system to study compartmentalised biochemical reactions.

Authors:  N Nuti; P E Verboket; P S Dittrich
Journal:  Lab Chip       Date:  2017-09-12       Impact factor: 6.799

7.  Real-time measurement of thrombin generation using continuous droplet microfluidics.

Authors:  Jiaqing Yu; Ding Tao; Ee Xing Ng; Chester L Drum; Ai Qun Liu; Chia-Hung Chen
Journal:  Biomicrofluidics       Date:  2014-09-03       Impact factor: 2.800

8.  Multiplexed protease activity assay for low-volume clinical samples using droplet-based microfluidics and its application to endometriosis.

Authors:  Chia-Hung Chen; Miles A Miller; Aniruddh Sarkar; Michael T Beste; Keith B Isaacson; Douglas A Lauffenburger; Linda G Griffith; Jongyoon Han
Journal:  J Am Chem Soc       Date:  2012-11-29       Impact factor: 15.419

9.  Functional profiling of circulating tumor cells with an integrated vortex capture and single-cell protease activity assay.

Authors:  Manjima Dhar; Jeffrey Nam Lam; Tonya Walser; Steven M Dubinett; Matthew B Rettig; Dino Di Carlo
Journal:  Proc Natl Acad Sci U S A       Date:  2018-09-17       Impact factor: 11.205

10.  Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions.

Authors:  Michael Ryckelynck; Stéphanie Baudrey; Christian Rick; Annick Marin; Faith Coldren; Eric Westhof; Andrew D Griffiths
Journal:  RNA       Date:  2015-01-20       Impact factor: 4.942
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