Literature DB >> 19140727

Using NMR to distinguish viscosity effects from nonspecific protein binding under crowded conditions.

Conggang Li1, Gary J Pielak.   

Abstract

Conventional NMR approaches to detect weak protein binding and aggregation are hindered by the increased viscosity brought about by crowding. We describe a simple and reliable NMR method to distinguish viscosity effects from binding and aggregation under crowded conditions.

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Year:  2009        PMID： 19140727      PMCID： PMC2645536          DOI： 10.1021/ja808428d

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  21 in total

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Review 4.  Macromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences.

Authors:  Huan-Xiang Zhou; Germán Rivas; Allen P Minton
Journal:  Annu Rev Biophys       Date:  2008       Impact factor: 12.981

5.  Self-diffusion and self-association of lysozyme molecules in solution.

Authors:  I V Nesmelova; V D Fedotov
Journal:  Biochim Biophys Acta       Date:  1998-04-02

6.  A pulsed-field gradient NMR study of bovine pancreatic trypsin inhibitor self-association.

Authors:  E Ilyina; V Roongta; H Pan; C Woodward; K H Mayo
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7.  Effects of molecular crowding on protein self-association: a potential source of error in sedimentation coefficients obtained by zonal ultracentrifugation in a sucrose gradient.

Authors:  J R Cann; R O Coombs; G J Howlett; M P Jacobsen; D J Winzor
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8.  Effect of macromolecular crowding upon the structure and function of an enzyme: glyceraldehyde-3-phosphate dehydrogenase.

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9.  Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli.

Authors:  S B Zimmerman; S O Trach
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10.  Detection of insulin aggregates with pulsed-field gradient nuclear magnetic resonance spectroscopy.

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  24 in total

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Review 6.  Soft interactions and crowding.

Authors:  Mohona Sarkar; Conggang Li; Gary J Pielak
Journal:  Biophys Rev       Date:  2013-02-21

Review 7.  Effects of macromolecular crowding agents on protein folding in vitro and in silico.

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8.  Volume exclusion and soft interaction effects on protein stability under crowded conditions.

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9.  Exploring weak, transient protein--protein interactions in crowded in vivo environments by in-cell nuclear magnetic resonance spectroscopy.

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10.  Probing non-specific interactions of Ca²⁺-calmodulin in E. coli lysate.

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