Feng Qin1, Xi Huang, Hong-Min Zhang, Ping Ren. 1. Laboratory of Ethnopharmacology and Department of Integrated Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, China.
Abstract
OBJECTIVES: An investigation has been designed and conducted to compare the pharmacokinetics of puerarin after oral administration of Jiawei-Xiaoyao-San to healthy volunteers and to patients with functional dyspepsia. METHODS: Quantification of puerarin in serum was achieved using a simple and rapid HPLC method for pharmacokinetic study. KEY FINDINGS: After oral administration of decoctions of Jiawei-Xiaoyao-San to healthy volunteers and patients with functional dyspepsia, puerarin was absorbed and reached a maximum concentration of 56.47+/-15.60 and 29.40+/-8.89 ng/ml at 51.00+/-8.22 and 50.00+/-12.25 min, respectively. Compared with the value of AUC0-360 (5.55+/-1.47 microg/ml min) in healthy volunteers, a smaller value of AUC0-360 (3.35+/-1.17 microg/ml min) was observed in patients with functional dyspepsia. CONCLUSIONS: There were statistically significant differences in the pharmacokinetic parameters of puerarin including the values for Cmax, AUC0-360, Cl/F and MRT0-360 between healthy volunteers and patients with functional dyspepsia. The pharmacokinetic parameters showed that functional dyspepsia reduced the absorption of puerarin after oral administration of Jiawei-Xiaoyao-San.
OBJECTIVES: An investigation has been designed and conducted to compare the pharmacokinetics of puerarin after oral administration of Jiawei-Xiaoyao-San to healthy volunteers and to patients with functional dyspepsia. METHODS: Quantification of puerarin in serum was achieved using a simple and rapid HPLC method for pharmacokinetic study. KEY FINDINGS: After oral administration of decoctions of Jiawei-Xiaoyao-San to healthy volunteers and patients with functional dyspepsia, puerarin was absorbed and reached a maximum concentration of 56.47+/-15.60 and 29.40+/-8.89 ng/ml at 51.00+/-8.22 and 50.00+/-12.25 min, respectively. Compared with the value of AUC0-360 (5.55+/-1.47 microg/ml min) in healthy volunteers, a smaller value of AUC0-360 (3.35+/-1.17 microg/ml min) was observed in patients with functional dyspepsia. CONCLUSIONS: There were statistically significant differences in the pharmacokinetic parameters of puerarin including the values for Cmax, AUC0-360, Cl/F and MRT0-360 between healthy volunteers and patients with functional dyspepsia. The pharmacokinetic parameters showed that functional dyspepsia reduced the absorption of puerarin after oral administration of Jiawei-Xiaoyao-San.
Authors: Alfonso Baldi; Antonio De Luca; Patrizia Maiorano; Costantino D'Angelo; Antonio Giordano Journal: Int J Mol Sci Date: 2020-03-07 Impact factor: 5.923