Literature DB >> 1911413

Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQ alpha gene.

J M Jung1, C T Comey, D B Baer, B Budowle.   

Abstract

A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQ alpha gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.

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Year:  1991        PMID： 1911413     DOI： 10.1007/bf01369719

Source DB:  PubMed          Journal:  Int J Legal Med        ISSN： 0937-9827            Impact factor:   2.686


  6 in total

1.  A simple and sensitive method for quantifying human genomic DNA in forensic specimen extracts.

Authors:  J S Waye; L A Presley; B Budowle; G G Shutler; R M Fourney
Journal:  Biotechniques       Date:  1989-09       Impact factor: 1.993

2.  Hae III--a suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples.

Authors:  B Budowle; J S Waye; G G Shutler; F S Baechtel
Journal:  J Forensic Sci       Date:  1990-05       Impact factor: 1.832

3.  An evaluation of the polymerase chain reaction method for forensic applications.

Authors:  S A Westwood; D J Werrett
Journal:  Forensic Sci Int       Date:  1990-04       Impact factor: 2.395

4.  Mitochondrial DNA sequences from a 7000-year old brain.

Authors:  S Pääbo; J A Gifford; A C Wilson
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

5.  A potent inhibitor of Taq polymerase copurifies with human genomic DNA.

Authors:  R de Franchis; N C Cross; N S Foulkes; T M Cox
Journal:  Nucleic Acids Res       Date:  1988-11-11       Impact factor: 16.971

6.  Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.

Authors:  R K Saiki; P S Walsh; C H Levenson; H A Erlich
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205
  6 in total
  5 in total

1.  PCR-based typing of DNA extracted from cigarette butts.

Authors:  M N Hochmeister; B Budowle; J Jung; U V Borer; C T Comey; R Dirnhofer
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

2.  Extraction of high quality DNA from bloodstains using diatoms.

Authors:  S Günther; J Herold; D Patzelt
Journal:  Int J Legal Med       Date:  1995       Impact factor: 2.686

3.  Rapid discrimination among dermatophytes, Scytalidium spp., and other fungi with a PCR-restriction fragment length polymorphism ribotyping method.

Authors:  M Machouart-Dubach; C Lacroix; M F de Chauvin ; I Le Gall ; C Giudicelli; F Lorenzo; F Derouin
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

4.  Population data of the HLA DQ alpha locus in Dutch Caucasians. Comparison with other population studies.

Authors:  A D Kloosterman; B Budowle; E L Riley
Journal:  Int J Legal Med       Date:  1993       Impact factor: 2.686

5.  The best practice for preparation of samples from FTA®cards for diagnosis of blood borne infections using African trypanosomes as a model system.

Authors:  Heba A Ahmed; Ewan T MacLeod; Geoff Hide; Susan C Welburn; Kim Picozzi
Journal:  Parasit Vectors       Date:  2011-05-07       Impact factor: 3.876
  5 in total

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