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Literature DB >> 8664153

Extraction of high quality DNA from bloodstains using diatoms.

Abstract

A simple method is described for the extraction of high quality DNA for PCR amplification. The DNA was extracted by using Chelex-100 ion exchange resin or a special cell lysis buffer containing proteinase K. For further purification the DNA was bound to silica in the presence of a chaotrophic agent. Hence it is possible to unlimitedly wash the bound DNA and inhibitory substances are removed. By using diatoms as a source of silicates, this method is very economical and can therefore be used as a routine method.

Entities: Chemical

Mesh：

Substances：

Year: 1995 PMID： 8664153 DOI： 10.1007/bf01844828

Source DB: PubMed Journal: Int J Legal Med ISSN： 0937-9827 Impact factor: 2.686

9 in total

1. Rapid and simple method for purification of nucleic acids.

Authors: R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal: J Clin Microbiol Date: 1990-03 Impact factor: 5.948

2. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

Authors: P S Walsh; D A Metzger; R Higuchi
Journal: Biotechniques Date: 1991-04 Impact factor: 1.993

3. Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQ alpha gene.

Authors: J M Jung; C T Comey; D B Baer; B Budowle
Journal: Int J Legal Med Date: 1991 Impact factor: 2.686

4. Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver.

Authors: R C Allen; G Graves; B Budowle
Journal: Biotechniques Date: 1989 Jul-Aug Impact factor: 1.993

1 in total

1. The effects of DBH, MAOA, and MAOB on attentional biases for facial expressions.

Authors: Pingyuan Gong; Shoumin Xi; Guomin Shen; She Li; Peizhe Zhang; Guochang Cao; Fuchang Zhang; Yan Shen; Tiantian Feng; Hua Ma
Journal: J Mol Neurosci Date: 2012-10-05 Impact factor: 3.444

1 in total

Extraction of high quality DNA from bloodstains using diatoms.

1. Rapid and simple method for purification of nucleic acids.

2. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

3. Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQ alpha gene.

4. Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver.

5. DNA extraction from Pleistocene bones by a silica-based purification method.

6. Forensic validation of the STR systems SE 33 and TC 11.

7. Extraction of high quality genomic DNA from microsamples of human blood.

8. Rapid and efficient resolution of parentage by amplification of short tandem repeats.

9. Different types of structural variation in STRs: HumFES/FPS, HumVWA and HumD21S11.

1. The effects of DBH, MAOA, and MAOB on attentional biases for facial expressions.